Detection of Some resistance genes of Salmonella enterica subsp. Salamae and Salmonella enterica serotype Kentucky isolated from Turkey | ||||
| Benha Veterinary Medical Journal | ||||
| Article 13, Volume 38, Issue 1, March 2020, Page 66-69 PDF (272.47 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2020.20518.1138 | ||||
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| Authors | ||||
Alaa Gouda  1; Ashraf Abd El-Tawab2; Seham Homouda3
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| 1Veterinarian | ||||
| 2Bacteriology, Immunology and Mycology Dept., Faculty of Vet. Med., Benha University | ||||
| 3Food Hygiene Dept., Animal Health Research Institute, Tanta Branch, Egypt. | ||||
| Abstract | ||||
| The aim of this study was to determine the serotyping and antimicrobial resistance of isolated Salmonella from the appearently healthy turkey. A total of 150 (cloacal samples) from living apparently healthy turkeys samples were screened bacteriologically for the occurrence of Salmonella. A total of 6/150 (4%) isolates of Salmonella was recovered. Serotyping revealed two different serotype, S.Enterica subspp Salamae (33.33%) and S.Kentucky.(66.67%). The isolated Salmonella were highly resistant to ampicillin, cefaclor, cefotaxime, ceftazidime, amoxicillin/clavulanic acid(100%) followed by chloramphenicol and ciprofloxacin (83.3%) then gentamycin (66.67%) and azithromycin (33.3%). All isolates showed high sensitivity for imipenem. All strains are multidrug-resistance (MDR). Polymerase chain reaction (PCR) was applied on Salmonella isolates to detect resistance gene. Antibacterial resistance genes blaTEM, blaOXA, floR, aadB and qnrA were detected in (100%), (0%), (100%), (100%) and (0%) of tested Salmonella. A combination of genotypic and phenotypic markers can be useful in studying genetic variation among salmonellae populations in turkey production and delineating possible transmission pathways. Conclusion: This study demonstrates that appearently healthy turkeys could be a reservoir for Salmonella resistant to multiple antimicrobials and poses a serious public health threat. | ||||
| Keywords | ||||
| serotype; Salmonella; Turkey; Resistance gene | ||||
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