Proanthocyanidin ameliorates against ethanol -induced gastric mucosal erosion by attenuating inflammatory response, oxidative stress and apoptosis in rats | ||
| Benha Veterinary Medical Journal | ||
| Article 1, Volume 38, Issue 1, March 2020, Pages 1-8 PDF (1.06 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2020.22277.1151 | ||
| Authors | ||
| Reem Obaia* 1; Yakout Elsenosi2; Mohamed Mahfouz3; Samy Aziza4 | ||
| 1Veterinarian | ||
| 2Department of Biochemistry, Faculty of Veterinary Medicine , Benha University | ||
| 3Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt | ||
| 4Biochemistry Dep., Faculty of Veterinary Medicine, Benha University | ||
| Abstract | ||
| Gastric ulcer is a common chronic disease in human digestive system. Massive alcohol drinking can lead to gastric ulcer. The gastroprotective effect and molecular mechanisms of Proanthocyanidin in a rat model of ethanol-induced gastric mucosal erosion were investigated.35 male rats were divided into five equal groups. Group 1 :( Control normal) rats received no drugs. Group 2:(Early ulcer) rats received absolute ethanol (0.5ml/100g rat) orally on empty stomach and sacrificed one hour later. Group 3 :(Early ulcer + proanthocyanidin protected) rats received proanthocyanidin orally (300mg/kg b.wt/day) for 3 weeks before ethanol administration then sacrificed after one hour. Group 4: (Late ulcer) rats received ethanol similar to group 2 and sacrificed after 21 days. Group 5: (Late ulcer + proanthocyanidin treated) rats first administered with ethanol (0.5ml/100g rat) and after one hour proanthocyanidin was administered for 21 days. A significant increase in stomach L-MDA concentration with marked decrease in CAT activity and GSH concentration were observed in gastric erosion -induced rats. However, a significant depletion of gastric L-MDA level and marked increase in CAT activity and GSH concentration were observed after Proanthocyanidin treatment when compared to ulcerated rats. A significant up-regulation of gene expression level of BAX, NF-κB and IL-1β with down-regulation of Bcl-2 gene were observed in stomach of gastric erosion -induced rats. However, a significant down regulation of BAX, NF-κB and IL-1β with up-regulation of Bcl-2 gene were observed after Proanthocyanidin treatment. Conclusively, Proanthocyanidin protects rat gastric mucosa against ethanol-induced gastric erosion via anti-inflammatory, anti- apoptotic and anti-oxidative mechanisms. | ||
| Keywords | ||
| Proanthocyanidin; gastric erosion; oxidative stress; inflammatory mediators, apoptosis | ||
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