HPLC Standardization of The Methanolic Extract of Acrocarpus fraxinifolius leaves based on Gallic acid Content | ||
| Archives of Pharmaceutical Sciences Ain Shams University | ||
| Article 2, Volume 1, Issue 1, January 2017, Pages 5-8 PDF (695.09 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/aps.2017.10356 | ||
| Authors | ||
| Heba A.S. El-Nashar1; Omayma A. Eldahshan1; Abdel Nasser B. Singab* 2 | ||
| 1Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Abassia, 11566, Egypt | ||
| 2Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, 11566, Egypt. | ||
| Abstract | ||
| High-Performance Liquid Chromatography (HPLC) was developed for the standardization and quantification of gallic acid in the methanolic extract of Acrocarpus fraxinifolius Weight & Arn leaves. Linearity was observed in the range of 0.4–2 mg/mL with a correlation coefficient (r2) of 0.9978. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0054 mg/mL and 0.0163 mg/mL, respectively indicating the sensitivity of the applied method. Recovery values of 100.377 % indicate the best accuracy of the method. Gallic acid content was quantified as 168.75 ± 1.05 mg/g methanolic extract. This developed method was simple, accurate and precise to be used as a reference standard method for determination of gallic acid content in the plant extract. | ||
| Keywords | ||
| HPLC; Standardization; quantification; Gallic acid; Acrocarpus fraxinifolius; linearity; accuracy | ||
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