Partial Kinetic Analysis of Haemolymph Esterases From The Red Palm Weevil; Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae)
Fahmy, N., Amin, T. (2019). Partial Kinetic Analysis of Haemolymph Esterases From The Red Palm Weevil; Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae). EKB Journal Management System, 11(3), 169-180. doi: 10.21608/eajbsc.2019.104804
Nedal M. Fahmy; Tarek R. Amin. "Partial Kinetic Analysis of Haemolymph Esterases From The Red Palm Weevil; Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae)". EKB Journal Management System, 11, 3, 2019, 169-180. doi: 10.21608/eajbsc.2019.104804
Fahmy, N., Amin, T. (2019). 'Partial Kinetic Analysis of Haemolymph Esterases From The Red Palm Weevil; Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae)', EKB Journal Management System, 11(3), pp. 169-180. doi: 10.21608/eajbsc.2019.104804
Fahmy, N., Amin, T. Partial Kinetic Analysis of Haemolymph Esterases From The Red Palm Weevil; Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae). EKB Journal Management System, 2019; 11(3): 169-180. doi: 10.21608/eajbsc.2019.104804

Partial Kinetic Analysis of Haemolymph Esterases From The Red Palm Weevil; Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae)

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 15, Volume 11, Issue 3, December 2019, Pages 169-180    PDF (497.71 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2019.104804
Authors
Nedal M. Fahmy1; Tarek R. Amin2
11-Department of Pest Physiology, Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt 2-Department of Biology, University Collage of Taymaa, University of Tabouk (ut), Kingdom of Saudi Areabia(Ksa)
2Department of Pest Physiology, Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt
Abstract
The paper represents a comprehensive study of the optimum subcellular fractions were prepared from the haemolymph of the seventh instar of the red palm weevil (RPW), Rhynchophorus ferrugineus Oliv. (Coleopter Curculionidae) to study some physicochemical characteristics of haemolymph esterases. The results showed that there were differences in characters between haemolymph α-esterases (hydrolyze alpha naphthyl acetate) and β-esterases (hydrolyze beta naphthyl acetate). The optimal pH and temperature were 8 and 50°C for α-esterases, respectively, and they were 7 and 35°C for β-esterases, respectively. Km (Michaelis constant) determined with alpha naphthyl acetate was 4X10-7 M, and it was 13.3X10-6 M for that determined with beta naphthyl acetate. Vmax (maximum velocity) was 5.55 mg α-naphthol/min/mg protein and 1.66 mg β-naphthol/min/mg protein for α- and β-esterases, respectively. The effect of organic solvents on substrate hydrolysis was also discussed. The study provided biochemical optimized conditions for esterases activity, and with these ongoing studies, our further aim will be to develop new strategies for the red palm weevil control using disruptors of esterases as important detoxifying enzymes.
Keywords
Rhynchophorus ferrugineus; Haemolymph Esterases; kinetic properties
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