Value of Vitamin D Administration Versus Suppressing Gut Microbiota in Modifying Metabolic Associated Osteoarthritis in Rats | ||
| The Medical Journal of Cairo University | ||
| Article 105, Volume 88, March, March 2020, Pages 849-857 PDF (490.92 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjcu.2020.104897 | ||
| Authors | ||
| RANDA S. GOMAA, M.D.; JEHAN SAEED, M.D.; SAFYA E. ESMAEEL, M.D. | ||
| The Departments of Medical Physiology* and Internal Medicine**, Faculty of Medicine, Zagazig University | ||
| Abstract | ||
| Abstract Background: Metabolic syndrome (MetS) is considered as common risk factors for knee osteoarthritis (OA). Aim of Study: Comparing the effect of vit. D supplemen-tation and gut microbiota suppression on the development of metabolic knee OA in rats is the aim of the study. Material and Methods: Forty adult male rats were subdi-vided into control and high carbohydrate high fat (HCHF) fed rats that furtherly subdivided into: HCHF (positive control), HCHF-Vitamin D treated & HCHF-antibiotics treated. Body mass index (BMI), serum glucose, insulin, total cholesterol, triglycerides, HDL-C, LDL-C, inflammatory cytokines were estimated. Homeostatic model assessment for insulin resistance (HOMA-IR) was calculated. Synovial cytokine, tissue degen-erative enzymes gene expression and knee histopathology were assessed. Results: HCHF induced significant increase in BMI, glucose, insulin, HOMA-IR, serum and synovial cytokines and degenerative enzymes with dyslipidemia and knee joint damage compared with controls. Vit. D improved all parameters in comparison to HCHF fed group. Antibiotic administration improved glucose, insulin, HOMA-IR, dyslipidemia and serum cytokines but induced insignificant changes in BMI, synovial cytokines, degenerative enzymes and knee joint damage compared with HCHF fed group. Conclusion: HCHF diet led to development of knee joint damage associated with changes in the metabolic profile in rats. Vit D supplementation dramatically improved knee joint damage, obesity insulin resistance, dyslipidemia and inflam-mation systemically and locally while controlling gut dysbiosis by antibiotics did not improve obesity, local inflammation and knee damage but improved the other parameters less than Vit D. | ||
| Keywords | ||
| Inflammation; Metabolic syndrome; Obesity; Oxidative stress | ||
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