In vitro type 1 fimbriae expression and antibiotic resistance by uro-pathogenic bacterial isolates from Human Immunodeficiency Virus infected patients
Akinjogunla, O., Etukudo, I., Oshosanya, G., Onuh, P., Njowuishi, N. (2021). In vitro type 1 fimbriae expression and antibiotic resistance by uro-pathogenic bacterial isolates from Human Immunodeficiency Virus infected patients. EKB Journal Management System, 2(2), 243-251. doi: 10.21608/mid.2020.37655.1042
Olajide Akinjogunla; Idongesit Etukudo; Godwin Oshosanya; Peace Onuh; Nkemdilim Njowuishi. "In vitro type 1 fimbriae expression and antibiotic resistance by uro-pathogenic bacterial isolates from Human Immunodeficiency Virus infected patients". EKB Journal Management System, 2, 2, 2021, 243-251. doi: 10.21608/mid.2020.37655.1042
Akinjogunla, O., Etukudo, I., Oshosanya, G., Onuh, P., Njowuishi, N. (2021). 'In vitro type 1 fimbriae expression and antibiotic resistance by uro-pathogenic bacterial isolates from Human Immunodeficiency Virus infected patients', EKB Journal Management System, 2(2), pp. 243-251. doi: 10.21608/mid.2020.37655.1042
Akinjogunla, O., Etukudo, I., Oshosanya, G., Onuh, P., Njowuishi, N. In vitro type 1 fimbriae expression and antibiotic resistance by uro-pathogenic bacterial isolates from Human Immunodeficiency Virus infected patients. EKB Journal Management System, 2021; 2(2): 243-251. doi: 10.21608/mid.2020.37655.1042

In vitro type 1 fimbriae expression and antibiotic resistance by uro-pathogenic bacterial isolates from Human Immunodeficiency Virus infected patients

Microbes and Infectious Diseases
Article 9, Volume 2, Issue 2, May 2021, Pages 243-251    PDF (360.44 K)
Document Type: Original Article
DOI: 10.21608/mid.2020.37655.1042
Authors
Olajide Akinjogunla* 1; Idongesit Etukudo2; Godwin Oshosanya3; Peace Onuh4; Nkemdilim Njowuishi5
1Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria
22Department of Microbiology, Faculty of Biological Science, Abia State University, Uturu, Abia State, Nigeria
3Department of Medical Microbiology and Parasitology, University of Uyo, Uyo, Akwa Ibom State, Nigeria
4Department of Microbiology, Faculty of Science, University of Uyo, P.M.B. 1017, Uyo, Akwa Ibom State, Nigeria
5Department of Microbiology, Faculty of Science, University of Uyo, Akwa Ibom State, Nigeria
Abstract
Background: Somebacteria possess fimbriae that support their adherence to host cell surface, colonization of human epithelial cells and resistance to antibiotics. Aim: The expression of type 1 fimbriae and antibiotic resistance by isolates from HIV infected patients. Methods: Bacteriological analysis of mid-stream urine (MSU) samples of human immunodeficiency virus infected patients (n=61) was determined using microbiological technique. In vitro type 1 fimbriae expression and antibiotic susceptibility of bacteria was determined using human erythrocytes with D-mannose and disc diffusion technique. Results: Of the 61 MSU samples from subjects, 31.1% had positive bacterial growth ≥ 105 CFU/mL. Forty-nine (49) bacterial isolates, belonging to genera Staphylococcus, Escherichia, Pseudomonas, Streptococcus, Klebsiella, Enterococcus and Proteus were recovered from the MSU samples. Escherichia coli was predominant isolate with 26.5%, followed by S. aureus (18.4%), while E. faecalis had the lowest occurrence (4.1%). Of the 29 isolates exhibiting hemagglutinating activity, type 1 fimbriae was expressed by 16 isolates, while 13 isolates showed P-fimbriae. The results showed 18.4 % isolates exhibiting resistance to Gentamycin; between 30.6 % and 38.8% isolates were Tetracycline and Nitrofurantoin resistant, while ≤ 44.9% isolates were resistant to Chloramphenicol and Ampicillin. Proteus sp had a low level of resistance to Ciprofloxacin, while ≥ 46.2 % E. coli were resistant to Nitrofurantoin. There was significant relationship (p < 0.05) between isolates expressing type 1 fimbriae and resistance to Gentamycin, Nalidixic Acid and Ampicillin. Conclusion: This study has shown the phenotypic expression of type 1 fimbriae and antibiotic resistance by isolates from HIV infected patients.
Keywords
Fimbriae; Hemagglutination; Bacteria; HIV; Resistance
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