PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)
Kasem, S., Alkhalefa, N., Magouz, A., Ebrahem, B., Khodier, M., Abdo, W., Desouky, A. (2018). PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016). EKB Journal Management System, 16(1), 1-15. doi: 10.21608/kvmj.2018.110174
Samy Kasem; Noura Alkhalefa; Asmaa Magouz; Basma Ebrahem; Mohamed Khodier; Walied Abdo; Abdelrazek Desouky. "PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)". EKB Journal Management System, 16, 1, 2018, 1-15. doi: 10.21608/kvmj.2018.110174
Kasem, S., Alkhalefa, N., Magouz, A., Ebrahem, B., Khodier, M., Abdo, W., Desouky, A. (2018). 'PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)', EKB Journal Management System, 16(1), pp. 1-15. doi: 10.21608/kvmj.2018.110174
Kasem, S., Alkhalefa, N., Magouz, A., Ebrahem, B., Khodier, M., Abdo, W., Desouky, A. PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016). EKB Journal Management System, 2018; 16(1): 1-15. doi: 10.21608/kvmj.2018.110174

PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)

Kafrelsheikh Veterinary Medical Journal
Article 1, Volume 16, Issue 1, April 2018, Page 1-15  XML PDF (1.06 MB)
Document Type: Original Article
DOI: 10.21608/kvmj.2018.110174
View on SCiNiTO View on SCiNiTO
Authors
Samy Kasem email 1; Noura Alkhalefa1; Asmaa Magouz1; Basma Ebrahem1; Mohamed Khodier2; Walied Abdo3; Abdelrazek Desouky4
1Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
2Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
3Department of pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
4Department of Parasitology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
Abstract
Bovine ephemeral fever(BEF) suspected outbreaks recorded during the summer of (2013 to 2016)in the Delta provinces of Egypt. The aim of this study was the isolation and molecular characterization of BEF viruses detected from these outbreaks, as there is no currently information about BEFV in Egypt. Blood samples were collected from suspected cases andthe virus was isolated intracerebrallyin baby mice then passed to Madin Darby Bovine Kidney (MDBK) andAfrican green monkey kidney (VERO) cell lines. The isolated virus was identified by RT-PCR followed by sequencing of the glycoprotein (G) gene of BEFV isolate.The construction of phylogenetic tree based on genetic relationships of the BEFV isolate derived from Egypt and different countries was performed. The phylogenetic treeshowed that the Egyptian isolate was closely linked to the Israeli and Turkish isolates.
 

Highlights

 

 
Keywords
BEFV; Glycoprotein G gene; RT-PCR; sequencing; Egypt
Full Text

 

 
References
 

 
Statistics
Article View: 260
PDF Download: 366