AMELIORATIVE EFFECT OF LIPOXIN A4 ON TESTICULAR DAMAGE INDUCED BY HIND LIMB ISCHEMIA/REPERFUSION INJURY IN RATS: ROLE OF HMGB-1 | ||||
| Ain Shams Medical Journal | ||||
| Article 2, Volume 70, 4, 5 & 6, July 2019, Page 259-270 PDF (1.08 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asmj.2019.111142 | ||||
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| Authors | ||||
| Abeer A. Abo Zeid1; Noha M Shafik2 | ||||
| 1Physiology Department, Faculty of Medicine, Tanta University - Egypt. | ||||
| 2Biochemistry Department, Faculty of Medicine, Tanta University - Egypt. | ||||
| Abstract | ||||
| Background: Hind limb ischemia/reperfusion injury (I/R) inducing testicular damage is a medical urgency that requires urgent intervention. Aim of the work: to investigate involvement of HMGB1 and the effect of Lipoxin-A 4 (LX A4) on remote testicular injury caused by hind limb (I/R). Methods: 45 adult male Wister rats were divided into 3 equal groups; sham-operated group, untreated hind limb (I/R) group and LXA4 pretreated hind limb (I/R) group. Malondialdehyde (MDA), catalase activity, nitrite/ nitrate, tumor necrosis factor-alpha (TNF- α), caspase-3 activity and high mobility group box-1 protein (HMGB-1) concentration and its relative mRNA expression were measured in testicular tissue. Serum level of free testosterone was also measured. Histopathological examination of testicular tissue was done as well as caspase-3 activity. Results: The untreated hind limb (I/R) injury group showed significant increase in testicular MDA, nitrite/ nitrate, TNF-α, caspase-3, as well as relative mRNA expression and concentration of HMGB-1, with significant reduction of testicular catalase activity and serum free testosterone. These biochemical results were significantly reversed in LXA4 pretreated hind limb (I/R) group. In addition there was significant negative correlation between serum free testosterone level and either HMGB1 concentration and its relative mRNA expression, testicular MDA, nitrite/nitrate, TNF-α and caspase-3 activity and significant positive correlation with catalase activity. These results were corroborated by immunehistochemistry of caspase-3 and histopathological results of testicular tissue. Conclusion: HMGB1 has a role in pathophysiology in I/R remote injury of testis and LXA4may play an important ameliorative effect on it due to antioxidant, anti-inflammatory, antiapoptotic effects and its ability to increase NO production | ||||
| Keywords | ||||
| Lipoxin A4; HMGB protein 1; hind limb ischemia/ reperfusion; testis | ||||
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