EVALUATION OF COMMERCIAL PREBIOTIC PRODUCTS ON THE GROWTH OF SELECTED PROBIOTIC BACTERIA STRAINS | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 1, Volume 34, Issue 3, March 2009, Page 1709-1721 PDF (420.96 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2009.112154 | ||||
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| Authors | ||||
| I. H.I. Abd El-Ghany1; Sahar H. S. Mohamed2; Fatma A. M. Ramadan1 | ||||
| 1Dairy Science and Technology., Fac. of Agric. Cairo Univ. Giza, Egypt. | ||||
| 2Dairy Dept. National Research Center Dokki, Giza, Egypt. | ||||
| Abstract | ||||
| The commercial prebiotic in cereal- based products with bovine colostrum, namely Germa-Fit® and Extra-Fit® as culture media for the growth of selected probiotic lactic acid strains (grown separately): L.gasseria and L.rhamnosus were cultured in MRS (control) and commercial prebiotic products. The media were fermented for 48h at 37ºC and analysed for viable cell count and changes in pH values. In Germa-Fit® medium, both probiotic lactic acid strains were attained the highest population (9.6 cfu/ml) after 16h of fermentation time (P≤0.05). With the addition of lactose at 0.5, 1.0 and 1.5% the viability of L.gasseria was improved by approximately 2.1, 2.3 and 1.7 log10 cycles respectively, compared to 3.0, 2.3 and 2.5 for L.rhamnosus after 12h of fermentation time. The effect of Extra-Fit® medium was more positive for growth L.gasseria than that of L.rhamnosus after 8-24h of inoculation time. Generally, Germa-Fit® medium in the presence lactose exhibited the highest growth for both prebiotic strains compared to Extra-Fit® and MRS media. The changes in pH of commercial media were in the optimal pH range (4.5-4.4) for growth of lactic acid bacteria. Therefore, this study suggested that the feasibility use of commercial prebiotic products based- cereal with bovine colostrum in the place the widely used MRS for culture media of probiotic lactic acid bacteria to developing new synbiotic functional fermented dairy food products. | ||||
| Keywords | ||||
| prebiotic; media; growth; probiotic bacteria | ||||
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