Bacteriological and molecular studies on Salmonella species isolated from poultry farms | ||
| Benha Veterinary Medical Journal | ||
| Article 31, Volume 36, Issue 1, March 2019, Pages 280-293 PDF (189.45 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2019.114673 | ||
| Authors | ||
| Ashraf Abd El-Tawab1; Enas Abdelbaset1; Abd Elhalim Hegazy2; Rania Abd-Elmonem2 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Benha University, Egypt | ||
| 2Department of Poultry Disease, Animal Health Research Institute, Agriculture Research Center Giza, Egypt. | ||
| Abstract | ||
| This study comprise the examination of 100 samples of feed( 40), water (36) and litter (24) collected randomly from different chicken shops, houses and farms in Kafr El-Sheikh Governorate. Results of this survey revealed isolation of 14/100(14%) Salmonella species (3)(12.5%) isolates from litter, (5)(13.8%) isolates from water and (6)(25%) isolates from farms . The prevalence rate to Salmonella was (14%) Sensitivity test was made and isolates exhibit resistance against ciprofloxacin and cefotaxime with (89%) and highly sensitivity to amikacin with (100%) so amikacin is the first drug choice for treatment of Salmonella infection. A PCR based assay was developed to developed to detect the prevalence of Salmonella in samples and to evaluate plasmid and chromosome-borne virulence genes (stn-invA) which considered as a target genes for the detection of Salmonella The results of detection of resistance genes (qnr S-blaTEM) as a resistance genes in all isolates of Salmonella. | ||
| Keywords | ||
| Antibiogram; Feed; Resistance and virulence genes; Salmonella | ||
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