Bacteriological and molecular studies on Salmonella species isolated from poultry farms
Abd El-Tawab, A., Abdelbaset, E., Hegazy, A., Abd-Elmonem, R. (2019). Bacteriological and molecular studies on Salmonella species isolated from poultry farms. EKB Journal Management System, 36(1), 280-293. doi: 10.21608/bvmj.2019.114673
Ashraf Abd El-Tawab; Enas Abdelbaset; Abd Elhalim Hegazy; Rania Abd-Elmonem. "Bacteriological and molecular studies on Salmonella species isolated from poultry farms". EKB Journal Management System, 36, 1, 2019, 280-293. doi: 10.21608/bvmj.2019.114673
Abd El-Tawab, A., Abdelbaset, E., Hegazy, A., Abd-Elmonem, R. (2019). 'Bacteriological and molecular studies on Salmonella species isolated from poultry farms', EKB Journal Management System, 36(1), pp. 280-293. doi: 10.21608/bvmj.2019.114673
Abd El-Tawab, A., Abdelbaset, E., Hegazy, A., Abd-Elmonem, R. Bacteriological and molecular studies on Salmonella species isolated from poultry farms. EKB Journal Management System, 2019; 36(1): 280-293. doi: 10.21608/bvmj.2019.114673

Bacteriological and molecular studies on Salmonella species isolated from poultry farms

Benha Veterinary Medical Journal
Article 31, Volume 36, Issue 1, March 2019, Page 280-293  XML PDF (189.45 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2019.114673
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Authors
Ashraf Abd El-Tawab1; Enas Abdelbaset1; Abd Elhalim Hegazy2; Rania Abd-Elmonem2
1Department of Microbiology, Faculty of Veterinary Medicine, Benha University, Egypt
2Department of Poultry Disease, Animal Health Research Institute, Agriculture Research Center Giza, Egypt.
Abstract
This study comprise the examination of 100 samples of feed( 40), water (36) and litter (24) collected randomly from different chicken shops, houses and farms in Kafr El-Sheikh Governorate. Results of this survey revealed isolation of 14/100(14%) Salmonella species (3)(12.5%) isolates from litter, (5)(13.8%) isolates from water and (6)(25%) isolates from farms . The prevalence rate to Salmonella was (14%) Sensitivity test was made and isolates exhibit resistance against ciprofloxacin and cefotaxime with (89%) and highly sensitivity to amikacin with (100%) so amikacin is the first drug choice for treatment of Salmonella infection.  A PCR based assay was developed to developed to detect the prevalence of Salmonella in samples and to evaluate plasmid and chromosome-borne virulence genes (stn-invA) which  considered as a target genes for the detection of Salmonella The results of detection of resistance genes (qnr S-blaTEM) as a resistance genes in all isolates of  Salmonella.    
 
Keywords
Antibiogram; Feed; Resistance and virulence genes; Salmonella
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