EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN
El-Kon, I., Fattouh, E., Darwish, S., Farag, F. (2008). EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN. EKB Journal Management System, 6(2), 254-273. doi: 10.21608/kvmj.2008.116236
Ismail Ismail El-Kon; El-Sayed Mohammed Fattouh; Samy Anwer Darwish; Fady samir Farag. "EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN". EKB Journal Management System, 6, 2, 2008, 254-273. doi: 10.21608/kvmj.2008.116236
El-Kon, I., Fattouh, E., Darwish, S., Farag, F. (2008). 'EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN', EKB Journal Management System, 6(2), pp. 254-273. doi: 10.21608/kvmj.2008.116236
El-Kon, I., Fattouh, E., Darwish, S., Farag, F. EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN. EKB Journal Management System, 2008; 6(2): 254-273. doi: 10.21608/kvmj.2008.116236

EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN

Kafrelsheikh Veterinary Medical Journal
Article 12, Volume 6, Issue 2, October 2008, Page 254-273  XML PDF (695.7 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2008.116236
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Authors
Ismail Ismail El-Kon* 1; El-Sayed Mohammed Fattouh1; Samy Anwer Darwish2; Fady samir Farag3
1Theriogenology Department, Faculty of Veterinary Medicine, kafrelsheikh University, Kafr- Elsheikh -33516, Egypt.
2Mehallat Mousa Buffalo Research Station, Animal Production Research Institute, Ministry of Agriculture, Egypt.
3General Authority for Veterinary Services, Al-Gharbia, Province, Algarbia, Egypt.
Abstract
The present study was conducted to investigate the effect of semen extenders onthe characteristics of cooled buffalo semen and to determine the suitable extender which will give higher conception rate. Semen was collected from five buffalo (5-6 years) bulls twice weekly using artificial vagina. Freshly collected semen samples were evaluated and ejaculates of at least 70% individual motility and 6x106 sperm /ml were pooled, divided into three parts, diluted with tris egg yolk, citrate egg yolk or skimmed milk extender and cooled slowly to 5oC. Semen samples were evaluated for individual motility, alive sperm, plasma and acrosomal membranes integrity as well as viability index. Conception rates of buffalo cows artificially inseminated with cooled buffalo semen were also estimated. The results of the present study revealed that semen extender and storage time exert a highly significant effects on the characteristics of cooled buffalo semen with the higher values recorded for tris egg yolk diluted samples. The conception rate of cooled buffalo semen was significantly (P<0.01) higher in tris (70%) diluted samples than skimmed milk (60%) and egg yolk citrate (50%) extended samples. It was concluded that the tris egg yolk extender is the most satisfactory buffering system to improve the post-cooling semen quality in buffalo and gave the best conception rate.
Keywords
Buffalo; cooled semen; extender; fertility
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