Lycopene mitigates experimental colitis in rats by inhibiting oxidative stress-mediated inflammation and apoptosis | ||
| Benha Veterinary Medical Journal | ||
| Article 4, Volume 39, Issue 1, September 2020, Pages 16-21 PDF (286.34 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2020.37614.1236 | ||
| Authors | ||
| Hend Hashem* 1; Samy Ali Hussein2; Yakout El senosi3 | ||
| 1Biochemistry, veterinary medicine,benha university | ||
| 2Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt. | ||
| 3Biochemistry, veterinary medicine, benha university | ||
| Abstract | ||
| Objective: The potential protective and beneficial effects of Lycopene (LYC) in a rat model of acetic acid-induced ulcerative colitis (UC) were evaluated. Methods: Forty male albino rats were divided into five equal groups. Group I: (Control normal) rats received no drugs. Group II: (Early ulcerative colitis): rats received 2 ml (3% v/v) glacial acetic acid intracolonially at 21 th day of experiment and sacrificed 3 days later. Group III:(Early ulcerative colitis + Lycopene protected) rats received Lycopene (10 mg/kg body weight/day, orally) for 21 days prior to acetic acid administration. Group IV: (Late ulcerative colitis) rats received acetic acid similar to group II for 3 successive days and sacrificed after 21 days. Group V: (Late ulcerative colitis+ Lycopene treated) rats first administered with acetic acid then after 3 days Lycopene was administered for 21 days. Results: A significant increase in L-Malondialdehyde (L-MDA) with marked decrease in reduced glutathione (GSH) level and Catalase (CAT) activity were observed in colon tissue of UC-induced rats. Additionally, a significant up-regulation of nuclear factor kappa B (NF-κB), caspase-3 and Transforming growth factor-β1 (TGF-β1) and down-regulation of Interleukin -10 (IL-10) and Beta cell lymphoma-2 (Bcl-2) gene expression levels of were observed in colon of UC induced rats. However, a significant depletion of colon tissue L-MDA and down-regulation of NF-κB, caspase-3 and TGF-β1 in addition to marked increase in GSH concentration and CAT activity and up-regulation of IL-10 and Bcl-2 were observed after LYC treatment. Conclusions: Lycopene has powerful antioxidants, anti-inflammatory and anti-apoptotic effects against ulcerative colitis. | ||
| Keywords | ||
| Lycopene; Colitis; Oxidative stress; Anti-apoptotic and Inflammatory cytokine | ||
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