Isolation and identification of new sub-genotypes of virulent ND virus in broiler chickens in Qalybia governorate | ||
| Benha Veterinary Medical Journal | ||
| Article 30, Volume 39, Issue 1, September 2020, Pages 159-164 PDF (698.68 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2020.32932.1216 | ||
| Authors | ||
| Asmaa Ibrahim Desouky* 1; Ahmed Eissa Mohamed Saad2; Mohamed Abdelgide Elshorbagy3; Samah Abodalal4 | ||
| 1Avain and Rabbit disease department-veterinary medicine-benha univerisity | ||
| 2Avain and Rabbit disease department faculty of veterinary medicine benha university | ||
| 3Avain and Rabbit Diseases department faculty of veterinary medicine benha university | ||
| 4Newcastle disease department veterinary serum and Vaccine Researcher Institute, Agriculture Research Center, Abbasia, Cairo, Egypt | ||
| Abstract | ||
| A B S T R A C T Newcastle disease (ND) is a highly contagious disease causing huge economic losses to the poultry industry due to high morbidities and mortalities. This study was aimed to isolate and characterize the new sub-genotype NDV strains in Qalybia Governorate during 2017 and 2019. Sixty eight samples were collected from Sixty eight broiler chicken flocks suffering from high mortality reach to 80% with distinctive clinical signs and post-mortem lesions of NDV. NDV strains isolated through inoculation in specific pathogen free (SPF) eggs and were identified by hemagglutination (HA) and hemagglutiation inhibition(HI) techniques. There were 16/20 (about 80%) of selected suspected farm were positive for NDV With HI test . These hemagglutinating agents were confirmed as NDV strains through molecular characterization using one step RT-PCR targeting the partial F-gene of NDV. Sequencing and phylogenetic analysis were successfully performed with eight NDV isolates which possessed the motif 112 R/K-R-Q-K/RR116 at the C-terminus of the F2 protein and F (phenylalanine) at residue 117, indicating that they are velogenic genotype. The present study revealed the circulation of NDV class II genotype VII in Qalybia chicken flocks. | ||
| Keywords | ||
| Key words: Newcastle Disease; F protein Cleavage site; Genotype VII | ||
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