Statistical Optimization of Industrially Potent Streptomyces plicatus MK559563 Xylanase and Α-Amylase Production Utilizing Agricultural Wastes | ||
| Egyptian Journal of Microbiology | ||
| Article 11, Volume 55, Issue 1, 2020, Pages 137-152 PDF (2.68 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejm.2020.46345.1176 | ||
| Authors | ||
| Shaimaa Amer* 1; Faten Mostafa2 | ||
| 1Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt | ||
| 2Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, 12622, Egypt | ||
| Abstract | ||
| Soil isolate Streptomyces plicatus MK559563 succeeded in the biological conversion of agricultural wastes (rice straw RS and potato shells PS) into valuable by-products (reducing sugar and industrial enzymes) by sub merged fermentation technique (SmF). Streptomyces plicatus MK559563 xylanase and α-amylase production were statistically optimized through two designs Plackett-Burman (PB) and Central composite design (CCD). PB design showed that xylanase production by S. plicatus MK559563 was more affected by incubation time, PS weight, and (NH4)2SO4 while α-amylase production was more affected by RS weight, (NH4)2SO4, and CaCl2. CCD improved S. plicatus MK559563 xylanase and α-amylase production by 39.69 and 61.60–fold increase compared to unoptimized media. The optimized media for S. plicatus MK559563 xylanase production composed of g/L: Baker’s yeast (1), (NH4)2SO4 (6.5), KCl (1), ZnSO4 (0.001) with RS (2g/flask) and PS (3.5g/flask) for 7 days of incubation. The optimized media for S. plicatus MK559563 α-amylase production composed of g/L: glucose (10), baker’s yeast (5), (NH4)2SO4 (6.5), CaCl2 (6.5) with RS (3.5g/flask) and PS (1g/flask) for 6 days of incubation. Streptomyces plicatus MK559563 can be used as an intermediate step for bioconversion of agricultural wastes into biofuel. | ||
| Keywords | ||
| α-amylase; Agricultural wastes; Statistical factorial design; Streptomyces sp; Xylanase | ||
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