Low Dose Gamma Radiation Induced Chromosomal Aberrations For the Management of the Green Vegetable Stink Bug, Nezara viridula (Hemiptera: Pentatomidae) | ||
| Egyptian Academic Journal of Biological Sciences. A, Entomology | ||
| Article 10, Volume 10, Issue 4 - Serial Number 1, June 2017, Pages 103-117 PDF (808.2 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsa.2017.12512 | ||
| Authors | ||
| Hatem A. M. Ibrahim; Dalia M. Mahmoud | ||
| Entomology Department, Faculty of Science, Ain Shams University, Cairo, Egypt. | ||
| Abstract | ||
| The impact of the low doses of ionizing radiation has been a matter of important consideration over the last few years. The present work investigates the chromosomal aberrations induced by two doses of radiations; a low dose as 10 Gy and a high dose as 60 Gy gamma radiation on the male germ cells of the green vegetable stink bug, Nezara viridula. The karyotype of the holocentric chromosomes at meiotic metaphase I for the non-irradiated and the irradiated adult males were examined by light microscope. Fourteen chromosomes were observed at the meiotic metaphase I of the non-irradiated spermatogonial cells. The typical diploid number of the 14 chromosomes was (12A + XY). Most of the cells showed six rod-like autosomal bivalent chromosomes and one pair of sex chromosomes. In some cells five to four bivalent rod-like with one or two ring chromosomes were recorded. Chromosomal aberrations were detected at meiotic metaphase I in the germ cells of the irradiated testes with 10 Gy dose. Translocation and aggregation (sticky) chromosomes with a tetravalent structure as well as B-Chromosomes were observed. Chromosomal fragmentations were also demonstrated in few gonial cells. At high dose as 60 Gy of gamma radiation, chromosomal fragmentations were only observed in the cells. The results confirm that low dose 10 Gy gamma radiation has induced chromosomal aberrations in the irradiated spermatogonial cells of the green vegetable stink bug, N. viridula. The present work provides part of the cytogenetic background necessary for the development of biological control protocols and future development of the sterile insect technique and support for the potential application of inherited partial sterility as an innovation in insect control against this economically important pest species. | ||
| Keywords | ||
| B-Chromosomes; Chromosomal aberration; Holocentric chromosomes; Low dose irradiation; Meiotic metaphase I; Nezara viridula | ||
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