ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA
Metwally, M., Abdel-Fattah, A., Abdel-Ghany, S., Geis, A., Heller, K., Okda, A. (2008). ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA. EKB Journal Management System, 33(11), 7997-8020. doi: 10.21608/jfds.2008.125183
M. M. Metwally; A. M. Abdel-Fattah; S. Abdel-Ghany; A. Geis; K. J. Heller; A. Y. Okda. "ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA". EKB Journal Management System, 33, 11, 2008, 7997-8020. doi: 10.21608/jfds.2008.125183
Metwally, M., Abdel-Fattah, A., Abdel-Ghany, S., Geis, A., Heller, K., Okda, A. (2008). 'ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA', EKB Journal Management System, 33(11), pp. 7997-8020. doi: 10.21608/jfds.2008.125183
Metwally, M., Abdel-Fattah, A., Abdel-Ghany, S., Geis, A., Heller, K., Okda, A. ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA. EKB Journal Management System, 2008; 33(11): 7997-8020. doi: 10.21608/jfds.2008.125183

ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA

Journal of Food and Dairy Sciences
Article 9, Volume 33, Issue 11, November 2008, Page 7997-8020  XML PDF (1000.03 K)
Document Type: Original Article
DOI: 10.21608/jfds.2008.125183
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Authors
M. M. Metwally1; A. M. Abdel-Fattah1; S. Abdel-Ghany2; A. Geis3; K. J. Heller3; A. Y. Okda2
1Dairy Science and Technology Dept,, Fac. Agric.,Cairo Univ., Cairo, Egypt.
2Dairy Microbiology Lab, Food Industries and Nutrition Division, NRC, Cairo, Egypt.
3Federal Dairy Research Centre, Institute for Microbiology, Kiel, Germany.
Abstract
      Strain AK71, isolated from Egyptian fermented milk named Zabady, and identified as Enterococcus faecalis, produces a 4000 Da bacteriocin-like compound with inhibitory activity against Staphylococcus aureus 91161, Enterococcus faecalis 90519 and Serratia marcescens 98027. Bacteriocin AK71-like compound is inactivated by proteinase K but not when treated with catalase and trypsin. No change in activity was recorded after either 30 min or 2 h at pH values between 3.0 and 8.0, and after treatment at 100°C for 30 min. Activity was lost after treatment at 121°C for 20 min. The mode of action is bacteriostatic. The highest level of bacteriocin activity (51200 AU/ml) was recorded when cells were grown in MRS broth at 30°C for 12 h. Bacteriocin AK 71 shows activity towards Gram-negative bacteria making it different from  most bacteriocins described for Enterococcus spp.
Keywords
Bacteriocin AK71; Enterococcus faecalis
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