ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA | ||
| Journal of Food and Dairy Sciences | ||
| Article 9, Volume 33, Issue 11, November 2008, Pages 7997-8020 PDF (1000.03 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jfds.2008.125183 | ||
| Authors | ||
| M. M. Metwally1; A. M. Abdel-Fattah1; S. Abdel-Ghany2; A. Geis3; K. J. Heller3; A. Y. Okda2 | ||
| 1Dairy Science and Technology Dept,, Fac. Agric.,Cairo Univ., Cairo, Egypt. | ||
| 2Dairy Microbiology Lab, Food Industries and Nutrition Division, NRC, Cairo, Egypt. | ||
| 3Federal Dairy Research Centre, Institute for Microbiology, Kiel, Germany. | ||
| Abstract | ||
| Strain AK71, isolated from Egyptian fermented milk named Zabady, and identified as Enterococcus faecalis, produces a 4000 Da bacteriocin-like compound with inhibitory activity against Staphylococcus aureus 91161, Enterococcus faecalis 90519 and Serratia marcescens 98027. Bacteriocin AK71-like compound is inactivated by proteinase K but not when treated with catalase and trypsin. No change in activity was recorded after either 30 min or 2 h at pH values between 3.0 and 8.0, and after treatment at 100°C for 30 min. Activity was lost after treatment at 121°C for 20 min. The mode of action is bacteriostatic. The highest level of bacteriocin activity (51200 AU/ml) was recorded when cells were grown in MRS broth at 30°C for 12 h. Bacteriocin AK 71 shows activity towards Gram-negative bacteria making it different from most bacteriocins described for Enterococcus spp. | ||
| Keywords | ||
| Bacteriocin AK71; Enterococcus faecalis | ||
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