EFFECTS OF SHORT RNA SEQUENCES ANALOGS AGAINST HCV GENOTYPE 4(A) IN REPLICON CELLS | ||
| Al-Azhar Journal of Pharmaceutical Sciences | ||
| Article 7, Volume 52, Issue 2 - Serial Number 52, September 2015, Pages 114-123 PDF (656.14 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajps.2015.12540 | ||
| Author | ||
| Ismail ELSHAFFEI | ||
| Department of Biochemistry, Faculty of Pharmacy, Misr International University, Cairo, Egypt | ||
| Abstract | ||
| Aim To determine to what extent short RNA sequences structural analogs could inhibit HCV genotype 4a (ED43) replication in cultured cell with sub-genomic HCV replicons. Method HCV short RNA sequences designed to be structurally similar to X region in the 3’-UTR of HCV 1b genome, were inserted into BamH I and Hind III restriction sites in pSilencer 4.1 CMV puro plasmid, transfected into human hepatoma cells Huh 7.5 with subgenomic replicons HCV genotype 4a (ED43/SG.Feo). 48 hrs after decoy transfection HCV RNA were isolated and quantified by real-time Polymerase chain reaction. Results the RNA structure analogs 5B46, X12, X12a and X12b were able to inhibit RNA levels by 65.5%, 38.5%, 64.5% and 68% respectively, in HCV genotype 4a. Conclusion Despite the sequence dissimilarity of the HCV RNA analogs and HCV genome in the replicon cells, there were enough 3 dimensional structural similarities to inhibit HCV viral replication. | ||
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