The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita
B., A., Shoaib, R., Taha, E., Ibrahim, S. (2016). The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita. EKB Journal Management System, 8(1), 49-59. doi: 10.21608/eajbsz.2016.13463
Abdel Razik A. B.; R. M. Shoaib; Entsar H. Taha; S. A. Ibrahim. "The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita". EKB Journal Management System, 8, 1, 2016, 49-59. doi: 10.21608/eajbsz.2016.13463
B., A., Shoaib, R., Taha, E., Ibrahim, S. (2016). 'The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita', EKB Journal Management System, 8(1), pp. 49-59. doi: 10.21608/eajbsz.2016.13463
B., A., Shoaib, R., Taha, E., Ibrahim, S. The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita. EKB Journal Management System, 2016; 8(1): 49-59. doi: 10.21608/eajbsz.2016.13463

The Effect of Serratia marcescens and Genetically Improved Pseudomonas fluorescens on Meloidogyne incognita

Egyptian Academic Journal of Biological Sciences, B. Zoology
Article 5, Volume 8, Issue 1, August 2016, Page 49-59  XML PDF (522.26 K)
Document Type: Original Article
DOI: 10.21608/eajbsz.2016.13463
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Authors
Abdel Razik A. B.1; R. M. Shoaib2; Entsar H. Taha3; S. A. Ibrahim4
1Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.
2Department of Genetics and Cytology, Genetic Engineering and Biotechnology Branch, National Research Center, Dokki, Egypt.
3Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Egypt
4Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Abstract
Pseudomonas fluorescens is an effective bacteria used in biocontrol agent for various soil borne plant diseases including plant parasitic nematodes. The aim of this study is enhancement the biocontrol effect of this bacterial strain by introducing chitinase gene(s) from Serratia marcescens. A DNA fragment containing the gene(s) were inserted into pGEM vector to construct a new plasmid, which introduced into P. fluorescens. Restriction enzyme digestion and chitin plate culture confirmed that the improved P. fluorescens is containing a functional chitinase gene(s). Bioassay comparative results among P. fluorescens wild type, Serratia marcescens and transformed P. fluorescens which conducted in screen house experiment indicated that transformed P. fluorescens had increased effect against plant parasitic nematodes (Meloidogyne incognita).  
Keywords
genetic transformation; Pseudomonas fluorescens; Serratia marcescens; chitinase gene; Meloidogyne incognita; biological control
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