Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef
M. Ebied, A., Abu-El-Regal, M., Abu-Almaaty, A., Mar`ie, Z. (2013). Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef. EKB Journal Management System, 36(2), 68-84. doi: 10.21608/djs.2013.139656
Abdel-Basset M. Ebied; M. A Abu-El-Regal; A. H Abu-Almaaty; Zeinab A Mar`ie. "Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef". EKB Journal Management System, 36, 2, 2013, 68-84. doi: 10.21608/djs.2013.139656
M. Ebied, A., Abu-El-Regal, M., Abu-Almaaty, A., Mar`ie, Z. (2013). 'Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef', EKB Journal Management System, 36(2), pp. 68-84. doi: 10.21608/djs.2013.139656
M. Ebied, A., Abu-El-Regal, M., Abu-Almaaty, A., Mar`ie, Z. Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef. EKB Journal Management System, 2013; 36(2): 68-84. doi: 10.21608/djs.2013.139656

Karyological and RAPD-PCR analysis of five marine fishes species ( Labridae-Perciformes ) From Red Sea coral reef

Delta Journal of Science
Article 11, Volume 36, Issue 2, June 2013, Pages 68-84    PDF (1.61 M)
Document Type: Research and Reference
DOI: 10.21608/djs.2013.139656
Authors
Abdel-Basset M. Ebied1; M. A Abu-El-Regal1; A. H Abu-Almaaty2; Zeinab A Mar`ie1
1Cytogenetic Laboratory-Zoology Department- Faculty of Science(Qena) - South Valley University- Egypt
2Zoology Department- Faculty of science- Port-said university
Abstract
Cytogenetic studies on five Red Sea reef fish species Larabicus quadrilineatus, Hemigymnus melapterus,
Thalassoma rueppellii, Thlassoma lunare and Gomphosus caeruleus of the family Labridae (Perciformes)
were carried out; all samples were collected from Hurghada, on the Egyptian Red Sea coast. All the
specimens showed a diploid chromosome number of 2n=48, they were also identical in the karyotype (all
acrocentric), and in the Fundamental number FN=48. The RAPD-PCR analysis were carried out by using
eight primers OPA12, 5`-TCGGCGATAG-3, OPA14,5`-TCTGTGCTGG-3`, OPA15, 5`-TTCCGAACCC-
3`, OPA17,5`- GACCGCTTGT – 3`, OPA18 5`-AGGTGACCGT-3`, OPA19 5`-CAAACGTCGG-
3`,OPA20,5`-GTTGCGATCC-3` and OPO11,5`- GACAGGAGGT- 3`.
The results were extremely useful to improve the importance of using RAPD-PCR in genetic analysis
among the species which are identical in chromosome number and karyotype.
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