Molecular Characterization of Fasciola spp. in Egypt on the Basis of certain rDNA fragments and Highly Repetitive DNA Sequences | ||
| Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology | ||
| Article 3, Volume 6, Issue 1, December 2014, Pages 19-28 PDF (309.46 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbse.2014.14500 | ||
| Author | ||
| Hoda A. Taha | ||
| Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||
| Abstract | ||
| The genetic identity of Fasciola species in Egypt has been investigated by carrying out the sequence analysis of the ITS-2 region of rDNA gene. Also, highly repetitive sequences of genomic DNA (124 bp) were determined and compared. Three isolates of Fasciola recovered from Egyptian local breeds (sheep, Buffaloes and Cows) were used. Sequence comparison including ITS-2 sequences of isolates from this study and reference sequences from Fasciola hepatica and F. gigantica and intermediate Fasciola in Genbank was done. The results revealed several variable sites encountered among the investigated isolates in comparison with species of Fasciola submitted in Genebank. The relationship between Egyptian Fasciola and Fasciola spp. from different countries were discussed. | ||
| Keywords | ||
| Fasciola spp. Egypt; ITS-2; Repetitive; DNA fragments; Sequencing | ||
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