Selective Cytotoxic and Chemoprotective effect of Epigallocatechine-3-Gallate against human HCT-116 cell line
Shehata, Y., Raafat, N., Hussien, M., Elsayed, E. (2019). Selective Cytotoxic and Chemoprotective effect of Epigallocatechine-3-Gallate against human HCT-116 cell line. EKB Journal Management System, 15(1), 196-205. doi: 10.21608/blj.2019.146624
Youssef M. Shehata; Nermin Raafat; Mohammed A. Hussien; Elsayed M. Elsayed. "Selective Cytotoxic and Chemoprotective effect of Epigallocatechine-3-Gallate against human HCT-116 cell line". EKB Journal Management System, 15, 1, 2019, 196-205. doi: 10.21608/blj.2019.146624
Shehata, Y., Raafat, N., Hussien, M., Elsayed, E. (2019). 'Selective Cytotoxic and Chemoprotective effect of Epigallocatechine-3-Gallate against human HCT-116 cell line', EKB Journal Management System, 15(1), pp. 196-205. doi: 10.21608/blj.2019.146624
Shehata, Y., Raafat, N., Hussien, M., Elsayed, E. Selective Cytotoxic and Chemoprotective effect of Epigallocatechine-3-Gallate against human HCT-116 cell line. EKB Journal Management System, 2019; 15(1): 196-205. doi: 10.21608/blj.2019.146624

Selective Cytotoxic and Chemoprotective effect of Epigallocatechine-3-Gallate against human HCT-116 cell line

Biochemistry Letters
Article 15, Volume 15, Issue 1, 2019, Pages 196-205
Document Type: Original Article
DOI: 10.21608/blj.2019.146624
Authors
Youssef M. Shehata1; Nermin Raafat2; Mohammed A. Hussien3; Elsayed M. Elsayed4
11. Professor of Biochemistry, Faculty of Veterinary Medicine, Zagazig University.
2Assistant Professor of Medical Biochemistry, Faculty of Medicine, Zagazig University.
3Assistant Professor of Biochemistry, Faculty of Veterinary Medicine, Zagazig University.
4B.V. SC , Faculty of Veterinary Medicine, Zagazig University
Abstract
Background: Epigallocatechin-3-gallate use as antioxidant and anticancer may related to its high   bioavailability.
 
Aim: this study was designed to investigate the possible chemoprotective and cytotoxic effect of Epigallocatechin-3-gallate against Human colorectal carcinoma (HCT-116) cell line.
Material and methods: MTT assay was performed to detect the cytotoxic effect of Epigallocatechine-3-Gallate against normal and HCT-116 cells, and determination of gene expression of VEGF,BFGF and Caspase-3 was performed to detect the apoptotic effect of Epigallocatechin-3-gallate
 Results: Epigallocatechin-3-gallate has a selective cytotoxic effect on human HCT-116 cells with no cytotoxic effect on healthy cells. At the same time, Epigallocatechine-3-Gallate increases the expression of Caspase-3 with reduction in expression of BFGF, VEGF gene .
 
Conclusion: Epigallocatechine-3-Gallate has selective cytotoxic and chemoprotective effect against human HCT-116 cells according to its dose used.
Keywords
Colorectal Cancer; HCT-116; Epigallocatechine-3-Gallate; MTTassay; BFGF; VEGF; Caspase-3
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