Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat
Saadati, A., Mashak, Z., Yarmand, M. (2021). Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat. EKB Journal Management System, 52(2), 163-173. doi: 10.21608/ejvs.2021.48755.1202
Amirhossein Saadati; Zohreh Mashak; Mohammad Saeid Yarmand. "Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat". EKB Journal Management System, 52, 2, 2021, 163-173. doi: 10.21608/ejvs.2021.48755.1202
Saadati, A., Mashak, Z., Yarmand, M. (2021). 'Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat', EKB Journal Management System, 52(2), pp. 163-173. doi: 10.21608/ejvs.2021.48755.1202
Saadati, A., Mashak, Z., Yarmand, M. Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat. EKB Journal Management System, 2021; 52(2): 163-173. doi: 10.21608/ejvs.2021.48755.1202

Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat

Egyptian Journal of Veterinary Sciences
Article 4, Volume 52, Issue 2, August 2021, Page 163-173  XML PDF (1.2 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2021.48755.1202
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Authors
Amirhossein Saadati1; Zohreh Mashak email 2; Mohammad Saeid Yarmand3
1Doctor Veterinary Medicine, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
2Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran.
3Department of Food Science and Engineering, Faculty of Agriculture, University of Tehran, Tehran, Iran.
Abstract
Enterotoxigenic methicillin-resistant Staphylococcus aureus (MRSA) is considered as one of the common foodborne pathogens. The existing research was performed to investigate the distribution of enterotoxigenic and antibiotic resistance genes of MRSA bacteria that are isolated from raw poultry meat samples. Two-hundred and sixty poultry meat samples were cultured and MRSA bacteria were recognized using cefoxitin and oxacillin susceptibility test. Antibiotic resistance and enterotoxigenic gene profiles were studied using PCR test. Prevalence of MRSA amongst poultry samples was 5%. Chicken (12%) had the highest prevalence rate, while ostrich (1.66%) had the lowest. Total distribution of sea, seb, sec, sed and see enterotoxin encoding genes were 50%, 25%, 8.33%, 75% and 8.33%, respectively. BlaZ (100%), aacA-D (58.33%), tetK (58.33%), msrA (58.33%) and dfrA1 (50%) were the most regularly detected antibiotic resistance-encoding genes. Concurrent presence of enterotoxins and antibiotic resistance-encoding genes in MRSA bacteria stipulates important public health matters regarding the consumption of contaminated chicken, turkey, quail and ostrich meat.


Keywords
Methicillin-resistant Staphylococcus aureus; Enterotoxigenic gene profile; Antibiotic resistance genes; Raw poultry meat
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