IN UTERO AND LACTATIONAL EXPOSURE TO DIOXIN ALTERED THE REPRODUCTIVE FUNCTION IN FEMALE RAT OFFSPRING BEFORE AND AFTER PUBERTY التعرض إلى الديوکسين عن طريق الرحم و الرضاعة يغير الوظيفة الإنجابية في ذرية الفئران الإناث قبل وبعد سن البلوغ | ||||
| Assiut University Bulletin for Environmental Researches | ||||
| Article 2, Volume 16.1, Issue 16.1, March 2013, Page 13-39 PDF (2.15 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/auber.2013.148457 | ||||
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| Abstract | ||||
| ABSTRACT: To evaluate effects of in utero and lactational 2,3,7,8-tetrachlorodibenzo-rho-dioxin (TCDD) exposure on the reproductive functionin female rat offspring, before and after puberty. The pregnant Sprague Dawely rat administered 0, or 1.0 mg TCDD/kg on Gestation Day (GD) 8 and 15. Female offspring were examined at the post-weanling before puberty on posnatal day (PND) 21 and in young adult stage of development on PND42. Ovulation assessment, radioimmunoassay for serum gonadotropins, steroids and histo-morphmetric analysis to the ovaries were evaluated. The analysis included a count, measurement and classification of preantral and antral follicles throughout the entire ovary on PND 21. The results indicate that TCDD treatment significantly reduced the ovulation rate, serum gonadotropins, steroids levels and the number of antral and preantral follicles of certain size classes. The histopathological examination revealed small preovulatory follicles displaying an atretic morphologic difference among the ovaries of rats exposed to TCDD treatments. These data support the hypothesis that TCDD results in adverse effects on female reproductive function. However, the age of animals before or after puberty play an important role in the difference between results. Moreover, TCDD exposure on the GD 8 or 15 has a great concern in the results observed. الملخص العربي : قد أجريت هذه الدراسة للتعرف علي إمکانية تقييم آثار التعرض للديوکسين عن طريق الرحم والرضاعة على الوظيفة الإنجابية في ذرية الفئران الإناث، قبل وبعد سن البلوغ. قد تم إعطاء الديوکسين للجرذان الحوامل بجرعات 0 أو 1,0 ميکروغرام / کغ في يوم الحمل الثامن و الخامس عشر. وقد بدا فحص ذرية الإناث في فترة ما بعد الفطام قبل سن البلوغ في اليومالواحد والعشرون بعد الولادة وأيضا تم فحص ذرية الإناث في مرحلة ما بعد البلوغ في اليومالثاني و الأربعين بعد الولادة. وقد تم تقييم لمستوي الإباضة، و أيضا وقد تم عمل تحليل کيميائي حيوي لقياس نشاط کل من الهرمونات الخاصة بالجونادوتروبين وأيضا قياس مستوي هرمون الاستروجين والبروجستيرون في المصل وذلک باستخدام المقايسة المناعية الشعاعية، کما تم فحص هستومورفولوجي للمبيضين. وشمل التحليل العد والقياس والتصنيف لکل أنوع البصيلات والجريبات الغارية في جميع أنحاء المبيض بالکامل. وتشير النتائج إلى أن التعرض إلى الديوکسين قد أدي إلي خفض معنوي ملحوظ فى معدل التبويض، وأيضا في مستوي الهرمونات الخاصة بالجونادوتروبين في المصل، وأيضا في مستويات هرمون الاستروجين والبروجستيرون في المصل، وقد أدي التعرض للديوکسين إلي نقصاً معنوياً ملحوظاً في عدد الجريبات الغارية والبصيلات من فئات معينة حجم عندما تم الفحص الهستومورفولوجي للمبيض. کما أظهر الفحص المجهرى للمبيض من ذرية الفئران المعرضة للديوکسين، تحلل وفقد و تغير فى شکل الخلايا التي تتکون منها أنسجة البصيلات المسئولة عن الإباضة. بالنظر لهذة النتائج يمکن أن نستنتج أن هذه البيانات تدعم الفرضية القائلة بأن التعرض للديوکسين قد يؤدي الي آثار ضارة على الوظيفة التناسلية للإناث. مع الوضع في الاعتبار، إن عمر الحيوانات قبل أو بعد سن البلوغ تلعب دوراً هاماً في الفرق بين النتائج. وعلاوة على ذلک، قد تم ملاحظة أن وقت التعرض للديوکسين أثناء الحمل في اليوم الثامن أو الخامس عشر کان له تأثير واضح للفرق بين النتائج. | ||||
| Keywords | ||||
| Keywords: Dioxin; Ovary; LH; FSH; Estrogen; Ovarian Follicles | ||||
| References | ||||
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