STUDIES ON DETECTION, DIAGNOSIS AND CONTROL OF LEGIONELLA PNEUMOPHILA IN DIFFERENT WATER SAMPLES
F.A., M., El-Sherbiny, S., El-Morsy, N. (2006). STUDIES ON DETECTION, DIAGNOSIS AND CONTROL OF LEGIONELLA PNEUMOPHILA IN DIFFERENT WATER SAMPLES. EKB Journal Management System, 14(2), 593-611. doi: 10.21608/ajs.2006.15304
Mansour F.A.; S.A. El-Sherbiny; N.A. El-Morsy. "STUDIES ON DETECTION, DIAGNOSIS AND CONTROL OF LEGIONELLA PNEUMOPHILA IN DIFFERENT WATER SAMPLES". EKB Journal Management System, 14, 2, 2006, 593-611. doi: 10.21608/ajs.2006.15304
F.A., M., El-Sherbiny, S., El-Morsy, N. (2006). 'STUDIES ON DETECTION, DIAGNOSIS AND CONTROL OF LEGIONELLA PNEUMOPHILA IN DIFFERENT WATER SAMPLES', EKB Journal Management System, 14(2), pp. 593-611. doi: 10.21608/ajs.2006.15304
F.A., M., El-Sherbiny, S., El-Morsy, N. STUDIES ON DETECTION, DIAGNOSIS AND CONTROL OF LEGIONELLA PNEUMOPHILA IN DIFFERENT WATER SAMPLES. EKB Journal Management System, 2006; 14(2): 593-611. doi: 10.21608/ajs.2006.15304

STUDIES ON DETECTION, DIAGNOSIS AND CONTROL OF LEGIONELLA PNEUMOPHILA IN DIFFERENT WATER SAMPLES

Arab Universities Journal of Agricultural Sciences
Article 4, Volume 14, Issue 2, September 2006, Page 593-611  XML PDF (1.01 MB)
Document Type: Original Article
DOI: 10.21608/ajs.2006.15304
View on SCiNiTO View on SCiNiTO
Authors
Mansour F.A.* 1; S.A. El-Sherbiny2; N.A. El-Morsy3
1Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
2Botany Department, Faculty of Science, Zagazig University, Zagazig, Egypt
3Digestive System Center, Mansoura University Hospital, Mansoura, Egypt
Abstract
A number of 192 samples (180 water & swab specimens and 12 patient sputa)
were chosen from different sites of Mansoura University Hospitals for detection and
diagnosis of Legionella pneumophila. Out of the 192 samples, 148 were positive by
culture method and non-specific staining technique such as gram staining and
biochemical analysis. In our trials for diagnosis of Legionella pneumophila, we
found that 107 of 148 samples were positive by slide agglutination test and 110
samples were positive by direct immunoflourescence assay, while 130 samples were
positive by polymerase chain reaction (PCR) technique which proved to be the most
specific and sensitive technique for diagnostic investigation of L. pneumophila. The
results show that the four disinfectant procedures (chlorine, ozone, U.V. light and
heat) were effective in eradicating Legionella pneumophila from different water
samples. Both UV light and heat (60ºC) produced a 5 log kill in less than 1 h. In
contrast, both chlorine and ozone required 5 h of exposure to produce a 5 log
decrease. Neither turbidity nor the higher temperature of 43ºC impaired the efficacy
of any of the disinfectant methods.
Keywords
Sputa; Legionella pneumophila; U.V. light; disinfectants; Immunoflourescence assay
Supplementary Files
Statistics
Article View: 173
PDF Download: 295