IN VITRO PROPAGATION OF SOMATIC EMBRYOGENESIS-DERIVED PLANTLETS OF CITRUS | ||||
| Arab Universities Journal of Agricultural Sciences | ||||
| Article 12, Volume 14, Issue 2, September 2006, Page 723-740 PDF (390.28 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajs.2006.15360 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| El-Sawy, A. A.* 1; A. Reda1; A. Gomaa2; Nansy Danial2 | ||||
| 1Plant Biotechnology Dep., Gen.Eng. and Bio. Div., National Research Center, Dokki, Egypt. | ||||
| 2Pomology Dep., Faculty of Agriculture, Cairo University, Giza, Egypt. | ||||
| Abstract | ||||
| Nodal stem segments of in vitro growing somatic embyogenesis and seedling-derived plantlets of sweet orange, C. sinensis (L.) of (Washington navel, shamouti and blood orange cvs.); grapefruit, C. paradisi (L.); Local mandarin, C. deliciosa (Tenor); lemon, C. limon; (L.); citron, C. medica (L.); lime, C. aurantifolia (Christm); rough lemon, C. jambhiri (Lush) and sour orange, C. aurantium (L.) were cultured on MS medium supplemented with different concentrations of BA (0.5,1.0 and 2.0 mg/L) alone or in combination with NAA (0.5 mg/L) for shoot multiplica-tion. The results indicated that somatic embryogenesis-derived plantlets can be suc-cessfully propagated in vitro with a good vigor compared with germinated seedlings. A large number of proliferated shoots were produced when these explants were cul-tured on MS medium containing 0.5mg/L of BA combined with 0.5mg/L of NAA. Most obtained shoots were rooted on half-strength MS medium containing 0.5 mg/L of NAA. These in vitro grown plantlets were then successfully transferred to green house through acclimatization process. | ||||
| Keywords | ||||
| citrus; In vitro propagation; Nodal segments; multiplication; Rooting | ||||
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