Improved Detection of Mycobacterium tuberculosis in Sudanese Children by use of Insertion Sequence IS6110
Osman, A., Saeed, N., Elmekki, M., Elhassan, M. (2014). Improved Detection of Mycobacterium tuberculosis in Sudanese Children by use of Insertion Sequence IS6110. EKB Journal Management System, 6(2), 65-70. doi: 10.21608/eajbsc.2014.16031
Ahmed L. Osman; Nageeb S. Saeed; Miskelyemen A. Elmekki; Mogahid M. Elhassan. "Improved Detection of Mycobacterium tuberculosis in Sudanese Children by use of Insertion Sequence IS6110". EKB Journal Management System, 6, 2, 2014, 65-70. doi: 10.21608/eajbsc.2014.16031
Osman, A., Saeed, N., Elmekki, M., Elhassan, M. (2014). 'Improved Detection of Mycobacterium tuberculosis in Sudanese Children by use of Insertion Sequence IS6110', EKB Journal Management System, 6(2), pp. 65-70. doi: 10.21608/eajbsc.2014.16031
Osman, A., Saeed, N., Elmekki, M., Elhassan, M. Improved Detection of Mycobacterium tuberculosis in Sudanese Children by use of Insertion Sequence IS6110. EKB Journal Management System, 2014; 6(2): 65-70. doi: 10.21608/eajbsc.2014.16031

Improved Detection of Mycobacterium tuberculosis in Sudanese Children by use of Insertion Sequence IS6110

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 7, Volume 6, Issue 2, December 2014, Page 65-70  XML PDF (319.95 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2014.16031
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Authors
Ahmed L. Osman1; Nageeb S. Saeed2; Miskelyemen A. Elmekki3; Mogahid M. Elhassan3
1Departments of Microbiology, College of Medical Laboratory Science, The National Ribat University, Khartoum, Sudan
2Department of Microbiology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
3-Departments of Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan. - College of Applied Medical Sciences, Taibah University, Al madenah Al monawarah, Kingdom of Saudi Arabia.
Abstract
This study was carried out in Khartoum state during the period from January 2010 to December 2013 to detect Mycobacterium tuberculosis in children with symptoms of tuberculosis infection using different conventional and advanced diagnostic techniques. One hundred ninety seven specimens of gastric lavage and sputum were collected from different hospital in Khartoum state.specimens were decontaminated and inoculated on Lowenstein Jensen media according to modified Pettrof’s method, two smears were prepared  and stained by Ziehl Neelsen stainbacterial DNA was extracted from each specimen by using phenol chloroform method, and then the Polymerase Chain Reaction technique was adopted to detect Insertion Sequence IS6110 gene of M. tuberculosisin in these specimens.
This study showed that the positive results for ZN, Culture and PCR were 16 (8.1%), 32 (16.2%) and 35(17.8%) respectively. The study concluded that the PCR technique is a most sensitive and specific technique for a fast identification of M. tuberculosis in gastric lavage and sputum from children who are unable to expectorate good quality sputum sample or diagnosed as negative using conventional diagnostic methods.
Keywords
IS 6110; ZN stain; MTB culture; Pediatric tuberculosis; Sudan
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