Modulation of autophagy enhances the apoptotic cell death in human T lymphoma cells treated with Anthocyanin | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 9, Volume 5, Issue 2, December 2013, Page 87-97 PDF (427.84 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2013.16098 | ||||
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| Authors | ||||
| Gamal H. El-Sokkary1; Douaa M. Sayed2; Salwa A. Fuad1; Abo Bakr M. Abdel Shakor1 | ||||
| 1Department of Zoology, faculty of Science, Assiut University, Assiut, 71516, Egypt | ||||
| 2Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, 71516, Egypt. | ||||
| Abstract | ||||
| In the present study, the effect of modulation of autophagy induced by anthocyanin on cell death of Human T lymphoma cells (Jurkat) was studied. Anthocyanin was abstracted from dry petals of Hibiscus sabdariffa, aquous solution of anthocyanin was added to cells at different concentrations then cell viability was determined by trypan blue exclusion method. Autophagy induced by anthocyanin was inhibited by 5 mM NH4CL which halts lysosomal enzymes and accordingly preventing autolysosomes formation. On the other hand, Autophagy was enhanced by glucose starvation. In both experiments of autophagy inhibition and enhancement, cell viability was studied to investigate the effect of autophagy modulation on cell viability. The results of this work revealed that both inhibition and enhancement of autophagy induced by anthocyanin lead to massive cell death. Immuno-detection of active caspase 3, one of the major hallmark of apoptotic cell death revealed remarkable increase of active caspse3 upon autophagy inhibition or enhancement. In conclusion, modulation of autophagy induced by anthocyanin lead to increasing of apoptotic cell death | ||||
| Keywords | ||||
| modulation of autophagy- human tlymphoma cells; Anthocyanin | ||||
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