IMMUNE RESPONSE OF HORSES VACCINATED WITH TISSUE CULTURE INACTIVATED EHV-1 OIL (MONTANIDE ISA-70) ADJUVANTED VACCINE | ||
| Egyptian Journal of Agricultural Research | ||
| Article 25, Volume 90, Issue 3, September 2012, Pages 1309-1321 PDF (359.71 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2012.163006 | ||
| Authors | ||
| SAFAA A. WARDA; GAMAL M. HUSSIEN | ||
| Veterinary Serum & Vaccine Research Institute, Abbasia, Cairo, Egypt. | ||
| Abstract | ||
| Tissue culture inactivated ISA-70 oil adjuvanted EHV-1 vaccine was successfully prepared from the local isolate. The virus was completely inactivated by 0.008 M of binary ethyleneimine (BEI) within 24 hours at 37ºC. Mineral oil (ISA-70) achieves long term immunity & good seringeabilty. The vaccine proved to be safe, immunogenic in the inoculated mice & pregnant mares. It was potent in vaccinated horses with protective level of neutralizing & complements fixing and AGPT antibodies for 5 months. The duration of virus recovery in vaccinated mice after challenge was 4 days compared with 9 days in the control group. The stability of the prepared vaccine was studied whereas it could be stored at 4ºC, and withstand room temperature and 37ºC for 2 years. | ||
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