An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time
Maharani, R., Amrullah, M., Kurnia, D., Hidayat, A., Nurlelasari, N., Harneti, D., Supratman, U. (2021). An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time. EKB Journal Management System, 64(9), 4797-4801. doi: 10.21608/ejchem.2021.52601.3085
Rani Maharani; Mufti Hanif Amrullah; Dessy Yulyani Kurnia; Ace Tatang Hidayat; Nurlelasari Nurlelasari; Desi Harneti; Unang Supratman. "An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time". EKB Journal Management System, 64, 9, 2021, 4797-4801. doi: 10.21608/ejchem.2021.52601.3085
Maharani, R., Amrullah, M., Kurnia, D., Hidayat, A., Nurlelasari, N., Harneti, D., Supratman, U. (2021). 'An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time', EKB Journal Management System, 64(9), pp. 4797-4801. doi: 10.21608/ejchem.2021.52601.3085
Maharani, R., Amrullah, M., Kurnia, D., Hidayat, A., Nurlelasari, N., Harneti, D., Supratman, U. An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time. EKB Journal Management System, 2021; 64(9): 4797-4801. doi: 10.21608/ejchem.2021.52601.3085

An Improved Synthesis of Pentapeptide (SCAP1e) Using Solid-Phase Method with Reduced Resin Loading Time

Egyptian Journal of Chemistry
Article 8, Volume 64, Issue 9, September 2021, Pages 4797-4801    PDF (272.59 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.52601.3085
Authors
Rani Maharani* 1; Mufti Hanif Amrullah2; Dessy Yulyani Kurnia3; Ace Tatang Hidayat2; Nurlelasari Nurlelasari4; Desi Harneti4; Unang Supratman1
1Department of Chemistry Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor 45363, Sumedang, West Java, Indonesia Central Laboratory Universitas Padjadjaran, Jl. Raya BandungSumedang Km 21 Jatinangor 45363, Sumedang, West Java, Indonesia
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran
3Department of Chemsitry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran
4Department of Chemistry Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21 Jatinangor 45363, Sumedang, West Java, Indonesia
Abstract
SCAP1e is an alanine-rich-pentapeptide, analog of SCAP1 an antioxidant peptide. The previous solid-phase synthesis on this peptide resulted in low yield of the product (7%) due to the aggregation that might occur during synthesis. The aim of the research was to resynthesize this peptide using the similar method but with a slight modification in the resin loading step. SCAP1e was successfully synthesized through solid-phase method with an improved yield 87.9% and high purity (90.2%) based on the HPLC analysis. The reduction of resin loading value from 1.2 mmol/gram to 0.63 mmol/gram has made a good impact for the improvement of the synthetic yield.
Keywords
Solid-phase peptide synthesis; resin loading; aggregation; SCAP1; SCAP1e
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