Determination of Trace Nitrite Amounts in Soil by Developed Cloud Point Extraction-Spectrophotometry Method | ||
| Egyptian Journal of Chemistry | ||
| Article 59, Volume 64, Issue 7, July 2021, Pages 3853-3859 PDF (437.65 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2021.61363.3325 | ||
| Authors | ||
| Ali Abdulwahid; Yossra Marich; Hasanain Abdulkareem; Hadi Al-Lami* | ||
| Department of Chemistry, College of Science, University of Basrah, Basrah 61001, Iraq | ||
| Abstract | ||
| A new and simple method for the selective and sensitive determination of trace amounts of nitrite in soil samples is developed by a combination of cloud point extraction (CPE) and spectrophotometry technique. The procedure is based on the Griess reaction on 3,3`-dimethyl benzidine with nitrite in the presence of 1-naphthol in acidic media to form a yellow diazo dye, which shows absorption at lambda max 455 nm. The formation of colored diazo dye is followed by extraction into the Triton X-114-rich phase. CPE method parameters such as surfactant amount, temperature, and incubation time are evaluated and optimized. The results showed a very good linear range of 10-1250 µgL-1, with good regression coefficients (R2) ≥ 0.9997 and a preconcentration factor of 12.5. The limit of detection (LOD) describes as 3SDblank/m, and the limit of quantification (LOQ) describes as 10SDblank/m, where ‘m’ is the slope of the calibration curve and they are equal to 7.50 and 22.71 µgL-1 respectively. The recommended method can apply to determine the ultra-trace amount of nitrite in soil samples. | ||
| Keywords | ||
| Nitrite; Spectrophotometric method; CPE; Griess reaction | ||
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