Bovine Serum Albumin Pre-concentration Using Glycidyl Methacrylate-co-ethylene Glycol Dimethacrylate-co- (2-diethylamino) ethyl Methacrylate Monolith | ||||
| Egyptian Journal of Chemistry | ||||
| Article 45, Volume 64, Issue 9, September 2021, Page 5137-5141 PDF (356.65 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2021.68171.3485 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Iman Abbas Hamza Khalaf; Ahmed Ali Alkarimi | ||||
| Department of Chemistry, College of Science, University of Babylon, Iraq | ||||
| Abstract | ||||
| Solid Phase Extraction (SPE) is one of the important as tool in the process of Pre-concentration of some biological compounds . such as proteins based on a solid support phase that interacts with the material to be Pre-concentration and then displaces that material with a suitable solvent in the lowest volume of the solvent . monolithic sorbent was prepared using a homogeneous mixture of two monomers Glycidyl methacrylate (GMA) and (2- diethylamino) ethyl methacrylate (2DEAMA) . In the presence of a cross-linker substance Ethelyn glycol dimethacrylate (EDMA) using a suitable solvent (porogenic solvent) consisting of 1-propanol and 2-butanol and a Initiator (2,2-dimethoxy-2-phenyl acetophenone) to the polymerization proces (photoelectric) . This monolithic was prepared inside a borosilicate tube . Evaluation of monolith performance was carried out by extraction of Bovine serum albumin standard protein and the extraction recovery was in the range (89 %) . | ||||
| Keywords | ||||
| : (2- diethylamino) ethyl methacrylate; solid-phase sorbent; glycidyl methacrylate; extraction | ||||
|
Statistics Article View: 495 PDF Download: 233 |
||||
