PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion
EL Naga, A., Shaltot, A., EL- Baz, A., fayad, E. (2008). PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion. EKB Journal Management System, 33(1), 577-586. doi: 10.21608/ejhm.2008.17620
Abo EL Naga; AM; Shaltot; AA; Rizk EL- Baz; Eman fayad. "PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion". EKB Journal Management System, 33, 1, 2008, 577-586. doi: 10.21608/ejhm.2008.17620
EL Naga, A., Shaltot, A., EL- Baz, A., fayad, E. (2008). 'PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion', EKB Journal Management System, 33(1), pp. 577-586. doi: 10.21608/ejhm.2008.17620
EL Naga, A., Shaltot, A., EL- Baz, A., fayad, E. PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion. EKB Journal Management System, 2008; 33(1): 577-586. doi: 10.21608/ejhm.2008.17620

PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion

The Egyptian Journal of Hospital Medicine
Article 11, Volume 33, Issue 1, October 2008, Page 577-586  XML PDF (403.27 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2008.17620
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Authors
Abo EL Naga1; AM; Shaltot2; AA; Rizk EL- Baz2; Eman fayad3
1Zoology Department, Faculty of Science at Mansoura University, Egypt.
2Genetics unit, children Hospital, Mansoura University, Egypt
3Genetics unit, children Hospital, Mansoura University, Egypt.
Abstract
Background: This study was conducted to test the utility of polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. T.gondii DNA was detected by using B1 gene as a target for amplification which is highly specific for T.gondii and is well conserved among all of the tested strains.
Results: This study revealed the following findings:(1) PCR was positive in 63 subjects, including 58 high risk cases (77.3%) and 5 of controls (12.5%). (2) 17 high risk cases (24.6%) had false positive IgM and 5 of controls (12.5%) had false negative result for IgM. (3) 17 high risk cases (32.7%) had false positive IgG and 5 of controls (12.5%) had false negative IgG. (4) No significant association between eating raw meat or contact with cats and positive ELISA for PCR but there is highly significant association between women with contact with soil and positive PCR. (5) No significant relation between residency and either ELISA or PCR. (6) Significant negative correlation between the age of the studied women and positivity of PCR.
Conclusion: this study highlights the need for a confirmatory test to detect primary acute toxoplasmosis in pregnant women. It demonstrates the possibility of defining and selecting the high-risk cases for mother-to-child transmission of infection by combining specific serology and PCR tests to formulate a specific approach
 
Keywords
PCR; Toxoplasma gondii and Repeated abortion
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