Second and Third Derivative Spectrophotometric Determination of Liothyronine and Thyroxine in Human Serum | ||
| Egyptian Journal of Chemistry | ||
| Article 23, Volume 64, Issue 11, November 2021, Pages 6385-6397 PDF (540.52 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2021.77986.3811 | ||
| Authors | ||
| Nabil A. Fakhre; Chnar M. Riasheed; Dashty Kh. Ali | ||
| Chemistry Department, College of Education, Salahaddin University, Erbil, Iraq | ||
| Abstract | ||
| A simple and accurate spectrophotometric method for simultaneous determination was used in this research of 3,3′,5-triidothyronine and thyroxine in human serum samples. The method created on the zero – crossing calculation for second and third derivative spectrophotometry. The calibration graphs are in the concentration spectrum, linear of 1.0 – 17.0 µgmL-1 and 1-18 µgmL-1 for T3 and T4 successively. The recoveries range from 93.78 – 102.68 % for T3 and 93.47 – 103.25 % for T4 with relative standard deviation less than 4.28% and 4.50% in all in stance for T3 and T4 respectively. | ||
| Keywords | ||
| 3; 5-triidothyronine; thyroxine; derivative spectrophotometry | ||
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