Study of the Pattern of Brucellosis in Menoufyia Governorate | ||||
| Afro-Egyptian Journal of Infectious and Endemic Diseases | ||||
| Article 5, Volume 5, Issue 2, June 2015, Page 86-93 PDF (341.08 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/aeji.2015.17817 | ||||
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| Authors | ||||
| Atef Abo EL-Soud Ali1; Ayman Mohammed El-Lehleh1; Somaia Abd El-Mohsen Shehab El-Deen1; Amira Abd El-Kader El-Hendy2; EL Sayed Mahmoud Mohammed Mousa1 | ||||
| 1Tropical Medicine Department, Faculty of Medicine, Menoufiya University ,Egypt | ||||
| 2Clinical Pathology Department, Faculty of Medicine, Menoufiya University, Egypt | ||||
| Abstract | ||||
| Background and study aim : ELISA can determine specific antibody classes against brucella, It is a sensitive, simple and rapid test, thus help to study the pattern of the brucellosis. The aim of this article is to study the pattern of the brucellosis in Menoufyia governorate. . Patients and Methods: Sera from 150 individuals from confirmed brucellosis cases and 25 healthy individuals were tested for presence of IgG and IgM antibodies by ELISA assay. Culture positivity for brucellosis was used as the reference standard for diagnosis. Results: Serum IgG and IgM for brucellosis by ELISA test had increased values in confirmed brucella cases, ELISA IgM was highly specific (100%) in all groups and sensitive (96%) in acute brucellosis, (100%) in subacute brucellosis and (64%) in chronic brucellosis. While, ELISA IgG specificity in all groups was (80%) and the sensitivity in acute brucellosis was (88%), in subacute and chronic brucellosis was (100%). Conclusion: ELISA IgG and IgM test for brucella is a simple and reliable test in the diagnosis of pattern of the brucellosis. | ||||
| Keywords | ||||
| Human brucellosis; ELISA IgG and IgM test for brucella; Serum samples | ||||
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