Optimization of Cultural Conditions to Maximize the Efficiency of Petroleum Crude Oil-Degrading Bacteria | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 8, Volume 10, Issue 1, June 2018, Page 85-94 PDF (403.83 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2018.17856 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Douaa H. Abdel-Aziz1; Sadia M. Easa2; Reda A. Abdel-Aziz Abdel-Aziz1; Said M. Badr El-Din1; Nevin A. Ibrahim2 | ||||
| 1Agricultural Microbiology Dept., National Research Center, Cairo, Egypt | ||||
| 2Microbiology Dept., Faculty of Science, Ain Shams University, Egypt | ||||
| Abstract | ||||
| The optimization of cultural conditions to maximize the efficiency of petroleum crude oil bioremediation by three bacterial strains, Alcaligenes faecalis, Microbacterium oxydans and Microbacterium Paraoxydans were isolated from Tebbin, Al Kanater charity and Agiba, respectively. The mineral salt medium was used, pH 7.5, temperature 30 oC and 30 days of incubation showed the highest degradation of crude oil with the isolated three bacterial strains. Ammonium sulphate as a sole N-source and crude oil as a sole C-sources were more suitable for crude oil degradation at the concentration of 0.2 % and 1.0 %, respectively. Inoculum size of 0.8 % was the most suitable inoculum for the degradation. Moreover, the agitation condition was advantages at 100 rpm than the static condition for the crude oil degradation by the three bacterial strains. | ||||
| Keywords | ||||
| Petroleum crude oil Alcaligenes faecalis Microbacterium oxydans; Microbacterium paraoxydans; biodegradation; strain efficiency; bioremediation; cultural conditions | ||||
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