RAPID DETECTION OF CONGO-CRIMEAN HAEMORRHAGIC FEVER ANTIBODIES | ||
| Assiut Veterinary Medical Journal | ||
| Article 22, Volume 39.2, Issue 78, July 1998, Pages 280-291 PDF (3.33 M) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.1998.183215 | ||
| Author | ||
| A.E. Agag | ||
| Dept. of Virology, Animal Health Research Institute, Dokki, Giza | ||
| Abstract | ||
| Serum sample from 3985 apparently healthy sheep and goats were tested for CCHF by indirect immunofluorescence (IF) and Dot-enzyme linked immunosorbent assay (Dot-ELISA). Infected inactivated Vero cell suspension was used as antigen in the study. CCHF viral antibody prevalence was found among sheep (2%), while not among goats. The and 13% in age >1 year and < percentages of reactive sera were 87% lyear subsequently. Neither IgM antibody in sera nor CCHF viral antigen in tick homogenates could be demonstrated. There was a correlation between Dot-ELISA and IF not only with the respect to the total numbers of positives, and negatives, but also, for each individual serum. Moreover, Dot-ELISA was more sensitive than IF, but needed more time for application. So IF technique was faster especially when rapid result delivery had been highly recommended. | ||
| Keywords | ||
| Key Words: Congo Fever; Crimean Haemorrhagic Fever; Antibodies | ||
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