RAPID DETECTION AND DIFFERENTIATION OF LEPTOSPIRA BY POLYMERASE CHAIN REACTION | ||
| Assiut Veterinary Medical Journal | ||
| Article 26, Volume 36.2, Issue 72, January 1997, Pages 326-335 PDF (2.78 M) | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.1997.183383 | ||
| Authors | ||
| G.S. RADWAN; E.R.H. ATTIA; M.S. TAWFIK | ||
| Abstract | ||
| The technique of arbitrarily primed polymerase chain reaction (AP-PCR) or random amplified polymorphic DNA (RAPD) fingerprinting was used for differentiation of leptospira. The assay allowed the rapid differentiation of standard: L.hardjo, L.pomona and L.canicola examined reference strains. In another experiment, this PCR assay successfully detected leptospiral DNA in L.hardjo-seeded bovine urine. The specificity of leptospiral DNA amplification from urine samples was confirmed by southern blot hybirdization using a nonradioactive labeled DNA probe. The data suggested that AP-PCR may be used for rapid differentiation and typing of leptospiral isolates, and a valuable test for specific detection of leptospira in bovine field samples. | ||
| Keywords | ||
| Key words: Leptospira; Detection; PCR | ||
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