Detection of Canine Parvovirus in Diarrheic Dogs in Three Egyptian Provinces During 2019-2020 | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 9, Volume 26, Issue 1, June 2021, Pages 123-130 PDF (981.36 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2021.184760 | ||
| Authors | ||
| Norhan Elsayed Salman* 1; Ahmed El-Sayed Mahmoud2; Eman El-Sayed3; Mokhtar El Tarabili4 | ||
| 1Veterinarian | ||
| 2Department of Animal Medicine, Faculty of Veterinary Medicine Suez Canal University, Ismailia, Egypt. | ||
| 3Department of Virology, Faculty of Veterinary Medicine, Suez Canal University | ||
| 4Department of virology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt | ||
| Abstract | ||
| Canine parvovirus(CPV2) is considered one of the serious and problematic diseases in young puppies, it remains a common and vital reason of morbidity and mortality in puppies, with very low survival rates in untreated dogs. It causes hemorrhagic enteritis and myocarditis in affected dogs. CPV2 has three antigenic variants CPV-2a, CPV-2b and CPV-2c—have been described, which are determined by variations at residue 426 of the VP2 capsid protein. The aim of the present study was to detect CPV-2 in feces of clinically diseased diarrheic puppies by rapid Immunochromatographic test (ICT) followed by polymerase chain reaction (PCR). One hundred fecal samples were collected from clinically suspected dogs with CPV-2 in three different provinces and test by ICT then make extraction of DNA and examined by PCR. The clinical diagnosis was confirmed in 45 suspected clinical cases (45 %) by rapid test (ICT) and 86 % by PCR using common and specific primers sets for detection of CPV2. | ||
| Keywords | ||
| Canine parvovirus 2 (CPV2); MVC minute canine virus; Epidemiology; Diagnosis; Treatment; PCR; rapid Immunochromatographic test (ICT) | ||
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