PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS | ||
| Zagazig Journal of Pharmaceutical Sciences | ||
| Article 6, Volume 7, Issue 2, December 1998, Pages 46-54 PDF (2.41 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjps.1998.184799 | ||
| Authors | ||
| Nehal Yousef* 1; Hemat Abd El-Latif2; Fathy Serry3; J. A. Cameron4 | ||
| 1Department of Microbiology, Faculty of Pharmacy, University of Zagazig - Egypt | ||
| 2Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt | ||
| 3Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt | ||
| 4Department of Microbiology, Molecular and Cell Biology, Connecticut University , Storrs, U.S.A | ||
| Abstract | ||
| In a previous screening study for polyhydroxy alkanoate (PHA) degrading activities, three isolates were obtained and characterized as Eikenella corrondens, Klebsiella pneumoniae and Alcaligenes faecalis . In the present study, PHA depolymerases from these three isolates were purified by DEAE anion exchange chromatography and by chromatofocusing ( isoelectric focusing ) after precipitation from the crude enzyme preparations by ammonium sulfate. The molecular weights for the three enzymes were determined by gel permeation sodium dodecyl sulphat (SDS) polyacrylamide gel eclectrophoresis. | ||
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