THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA
Timmis, K., Serry, F., Abd El-Latif, H., Chatwal, G., Shaker, G. (1995). THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA. EKB Journal Management System, 4(1), 7-17. doi: 10.21608/zjps.1995.186307
Kenneth Timmis; Fathy Serry; Hemat Abd El-Latif; Gursharan Chatwal; Ghada Shaker. "THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA". EKB Journal Management System, 4, 1, 1995, 7-17. doi: 10.21608/zjps.1995.186307
Timmis, K., Serry, F., Abd El-Latif, H., Chatwal, G., Shaker, G. (1995). 'THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA', EKB Journal Management System, 4(1), pp. 7-17. doi: 10.21608/zjps.1995.186307
Timmis, K., Serry, F., Abd El-Latif, H., Chatwal, G., Shaker, G. THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA. EKB Journal Management System, 1995; 4(1): 7-17. doi: 10.21608/zjps.1995.186307

THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA

Zagazig Journal of Pharmaceutical Sciences
Article 2, Volume 4, Issue 1, June 1995, Page 7-17  XML PDF (8.95 MB)
Document Type: Original Article
DOI: 10.21608/zjps.1995.186307
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Authors
Kenneth Timmis email 1; Fathy Serry2; Hemat Abd El-Latif3; Gursharan Chatwal4; Ghada Shaker5
1Gesellschaft Für Biotechnologische Forschung (GBF), Braunschweig, Germany.
2Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt
3Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt
4University of Braunschweig, Germany
5Department of Microbiology, Faculty of Pharmacy, Zagazig University, Egypt
Abstract
The binding of IgA with C-protein was studied and compared with that of jacalin. SDS- PAGE and Western blotting for various human IgA forms (IgA1, IgA2, serum IgA and secretory IgA) proved the specificity of C-protein binding and the superiority of biotinylated C-protein over biotinylated jacalin as probing immunochemical reagent for IgA detection. The obtained results proved the usefulness of employing C-protein / sepharose columns in isolation of IgA from the whole serum. Although, a linear quantitative relationship was established with pure IgA, the development of ELISA technique for quantification of IgA in whole serum was not possible in the present study. However, a proposed procedure was set for further work.
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