THE POTENTIAL APPLICATIONS OF C-PROTEIN AS IMMUNOREAGENT IN DETECTION, ISOLATION AND QUANTIFICATION OF IgA | ||
Zagazig Journal of Pharmaceutical Sciences | ||
Article 2, Volume 4, Issue 1, June 1995, Pages 7-17 PDF (8.95 M) | ||
Document Type: Original Article | ||
DOI: 10.21608/zjps.1995.186307 | ||
Authors | ||
Kenneth Timmis* 1; Fathy Serry2; Hemat Abd El-Latif3; Gursharan Chatwal4; Ghada Shaker5 | ||
1Gesellschaft Für Biotechnologische Forschung (GBF), Braunschweig, Germany. | ||
2Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt | ||
3Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt | ||
4University of Braunschweig, Germany | ||
5Department of Microbiology, Faculty of Pharmacy, Zagazig University, Egypt | ||
Abstract | ||
The binding of IgA with C-protein was studied and compared with that of jacalin. SDS- PAGE and Western blotting for various human IgA forms (IgA1, IgA2, serum IgA and secretory IgA) proved the specificity of C-protein binding and the superiority of biotinylated C-protein over biotinylated jacalin as probing immunochemical reagent for IgA detection. The obtained results proved the usefulness of employing C-protein / sepharose columns in isolation of IgA from the whole serum. Although, a linear quantitative relationship was established with pure IgA, the development of ELISA technique for quantification of IgA in whole serum was not possible in the present study. However, a proposed procedure was set for further work. | ||
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