The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 9, Volume 30, Issue 3, July 2021, Pages 65-69 PDF (341.63 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| Author | ||
| Marwa Nabil Mohamed* | ||
| General Directorate of Infection Control, Ministry of Health and Population | ||
| Abstract | ||
| Background: Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) is a novel technique for identification of microbes. This new method led to a new era in microbial identification because of its rapid, accurate, valid, simple and relatively decreased cost. Objectives: The aim of this study was identification of predominant pathogens by MALDI-TOF technique. Methodology: Pathogens were identified by both conventional methods and MALDI-TOF. Results: From July till December 2018, predominant pathogens were Klebsiella pneumoniae (21%), Pseudomonas aeroguinosa and Candida each constitutes (17%), E-coli (10%), Staph. aureus (9%), Acinetobacter (9%). Identification of isolates (from September to December 2018) by MALDI-TOF revealed a total agreement of (94.1%) with conventional method at genus level, (88.2%) at level of species. Kappa agreement revealed almost perfect correlation between both techniques. Conclusion: The MALDI-TOF results might suggest that its’ usage may be dependable for microbiological identification. | ||
| Keywords | ||
| MALDI -TOF; Microbiological Identification; Conventional Methods | ||
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