Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 12, Volume 30, Issue 3, July 2021, Pages 89-95 PDF (407.09 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| Author | ||
| Kareman Ahmed Eshra* | ||
| Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University | ||
| Abstract | ||
| Background: Acinetobacter species are very common in hospital acquired infections; Carbapenems resistant species become common in hospital and represent a major health problem. Methodology: Our study was carried on 150 patients from ICU; we compared different phenotypic methods and PCR for detection of carbapenems resistance in Acinetobacters. Results: 88.9% of resistant Acinetobacters were detected by Kirby Bauer Disk diffusion method, 54.2%by Modified Hodge test, 70.8 % by EDTA disc synergy, 85.4% by Amp C disk test, 33.3% by PCR bla-IMP and 12.5% by PCR bla-VIM. Conclusion: We recommended for detection of carbapenems resistance in Acinetobacters the phenotypic methods as they are rapid, accurate methods and less costly than PCR. Note: bla-IMP gene (NCBI GenBank Nucleotide accession # LC103138.1); bla-VIM gene (NCBI GenBank Nucleotide accession # MT185944.1 GenBank accession number S71932 | ||
| Keywords | ||
| Acinetobacters; Carbapenems resistance genes; Metallo beta lactamases | ||
|
Statistics Article View: 261 PDF Download: 241 |
||
