CYCLICAL CHANGES IN GONADOTROPIC CELLS IN RELATION TO THE OVARIAN ACTIVITY IN BUFFALOES | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 2, Volume 17.1, Issue 33, May 1986, Page 14-21 PDF (2.62 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.1986.189960 | ||||
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| Authors | ||||
| A.H.S. HASSAN; A. ABOUL-ELA; M.R. FATH EL-BAB; AZIZA A. SELIM | ||||
| Abstract | ||||
| The gonadotropic cells within the pars distalis of 33 mature female buffaloes, including normal cycling animals and animals suffering from ovarian inactivity were histochemically investigated. Using performic acid-Alcian blue-periodic acid Schiff-orange G (PEA-AB-PAS-OG) stain, the FSH- and LH- cells were differentiated and identified. The FSH and LH pituitary contents were also immunologically assayed. During Proestrus, the FSH-cells were increased in size and number and constituted about 14.4% of the total cell count. The FSH/LH ratio was 1.62. During estrus, the LH-cells were highly increased in amount (12.2%) and the FSH/LH ratio was 0.26. In diestrus, the FSH-cells percentage was 12.6%, while the LH-cell percentage was 10.4% with a FSH/LH ratio of 0.65. In animals affected with inactive ovaries, two contrastic data could be obtained. These animals either show a high FSH cell % and content with a negligible LH cell % and content, or a high LH cell % and content with a negligibel FSH cell % and content. It can concluded that for proper treatment of buffaloes suffering from inactive ovaries, the FSH/LH ratio should be restored to that ratio at proestrus. | ||||
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