Performance of quantum dot nanobeads-based immunoassay in diagnosis of toxoplasmosis
Abd El-Ghani, H., Abdel-Salam, I., Ali, I., Anwar, M., Shams El-Din, H., Sabry, N., Mady, R. (2021). Performance of quantum dot nanobeads-based immunoassay in diagnosis of toxoplasmosis. EKB Journal Management System, 14(2), 204-213. doi: 10.21608/puj.2021.83445.1123
Hamssa Abd El-Ghani; Iman Abdel-Salam; Ibrahim Ali; Mona Anwar; Hala Shams El-Din; Nadia Sabry; Rasha Mady. "Performance of quantum dot nanobeads-based immunoassay in diagnosis of toxoplasmosis". EKB Journal Management System, 14, 2, 2021, 204-213. doi: 10.21608/puj.2021.83445.1123
Abd El-Ghani, H., Abdel-Salam, I., Ali, I., Anwar, M., Shams El-Din, H., Sabry, N., Mady, R. (2021). 'Performance of quantum dot nanobeads-based immunoassay in diagnosis of toxoplasmosis', EKB Journal Management System, 14(2), pp. 204-213. doi: 10.21608/puj.2021.83445.1123
Abd El-Ghani, H., Abdel-Salam, I., Ali, I., Anwar, M., Shams El-Din, H., Sabry, N., Mady, R. Performance of quantum dot nanobeads-based immunoassay in diagnosis of toxoplasmosis. EKB Journal Management System, 2021; 14(2): 204-213. doi: 10.21608/puj.2021.83445.1123

Performance of quantum dot nanobeads-based immunoassay in diagnosis of toxoplasmosis

Parasitologists United Journal
Article 13, Volume 14, Issue 2, August 2021, Page 204-213  XML PDF (505.5 K)
Document Type: Original Article
DOI: 10.21608/puj.2021.83445.1123
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Authors
Hamssa Abd El-Ghani1; Iman Abdel-Salam1; Ibrahim Ali2; Mona Anwar1; Hala Shams El-Din* 1; Nadia Sabryorcid 1; Rasha Mady3
1Departments of Medical Parasitology Faculty of Medicine, Ain Shams
2Immunoparasitology and Evaluation of drugs, Theodor Bilharz Research Institute , Egypt
3Departments of Medical Parasitology Faculty of Medicine , Alexandria Universities
Abstract
Background: Diagnosis of toxoplasmosis relies mainly on determination of IgG and IgM using ELISA.
However, inter-laboratory evaluation of performance of many commercial kits gave unsatisfactory
agreements. Merging the innovative use of quantum dots nanobeads (QDNBs) immunoassay may yield
higher sensitivity and specificity than the conventional used methods.
Objective: The present study aims to evaluate the performance of QDNBs based immunoassay in
serodiagnosis of human toxoplasmosis.
Subjects and Methods: The study included 175 subjects divided into three categories; 122 patients
with clinical manifestations suggestive of toxoplasmosis; 32 patients proved positive for other parasitic
infections, and 21 healthy control subjects. In comparison to Sabin Feldman dye test (SFDT), as a gold
standard test, serum samples were examined in an immunoassay utilizing QDNBs for detection of T. gondii
specific IgG and IgM antibodies.
Results: Compared to SFDT, combined QDNBs-IgG/IgM immunoassay exhibited sensitivity of 95.4%,
specificity of 98.9%, positive predictive value (PPV) of 98.8%, and negative predictive value (NPV) of
95.6%, with relative agreement, validity, and accuracy of almost 97%.
Conclusion: It was concluded that QDNBs-based immunoassay improves the performance of ELISA
particularly sensitivity as well as shorten the assay time.
Keywords
diagnosis; human toxoplasmosis; immunoassay; quantum dots; T. gondii
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