Influence of Intracanal Cryotherapy on Inflammatory Mediators Expression using Different Irrigation Protocols: A Randomized Clinical Trial | ||
| Dental Science Updates | ||
| Article 7, Volume 2, Issue 2, September 2021, Pages 165-174 PDF (1.08 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/dsu.2021.58237.1063 | ||
| Authors | ||
| Ahmed Emad Mourad* 1; Nelly Abdelsalam2; Dalia Fayyad3 | ||
| 1Ph.D candidate , faculty of dentistry, suez canal university | ||
| 2Department of Endodontics ,Suez Canal University, Faculty of Dentistry, Egypt. | ||
| 3Dean of Faculty of Dentistry, Suez Canal University, Egypt. | ||
| Abstract | ||
| ABSTRACT Aim: To evaluate the effect of intra-canal cryotherapy on interleukin-1β and interleukin-10 expression in teeth with symptomatic apical periodontitis. Methodology: Forty-eight single rooted teeth in patients with symptomatic apical periodontitis were randomly divided into 4 groups according to the irrigation protocol. Group I (control); 5% sodium hypochlorite at room temperature. Group II; 5% cold sodium hypochlorite (2-5°C). Group III; final rinse with 20 mL of 5% cold sodium hypochlorite (2-5°C) for 5 minutes. Group IV; final rinse with 20 mL of cold saline (2- 5°C) for 5 minutes. Paper point passing 2 mm beyond the apex was used to collect periapical fluid before, immediately after mechanical instrumentation and after one week to characterize the mRNA expression of IL-1β and Il-10 using real-time polymerase chain reaction (PCR). Statistical analysis was performed using one-way ANOVA to compare between all groups. Results: Our results revealed statistically significant (at P≤ 0.05) down regulation of IL-1β gene expression levels in all groups after one week with no statistically significant difference between the four groups and statistically significant (at P≤ 0.05) up regulation of IL-10 gene expression levels in all groups after one week. Conclusion: All the cryotherapy irrigation protocols showed lower levels of expression of the pro-inflammatory mediators (interleukin-1β) and higher levels of expression of the anti-inflammatory mediator (interleukin-10) than the control group after one week. | ||
| Keywords | ||
| Cryotherapy; Interleukins; PCR; Sodium hypochlorite | ||
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