Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast
rashad, S., abdel-tawab, F., fahmy, E., Attallah, A., Ahmed, E., haggran, A. (2021). Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast. EKB Journal Management System, 64(12), 7649-7667. doi: 10.21608/ejchem.2021.90428.4315
shimaa elsayed rashad; fatthy mohamed abdel-tawab; eman mahmoud fahmy; Ashraf Gamil Attallah; Ekram Sallah Eldin Ahmed; abdelhamed abdelalem haggran. "Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast". EKB Journal Management System, 64, 12, 2021, 7649-7667. doi: 10.21608/ejchem.2021.90428.4315
rashad, S., abdel-tawab, F., fahmy, E., Attallah, A., Ahmed, E., haggran, A. (2021). 'Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast', EKB Journal Management System, 64(12), pp. 7649-7667. doi: 10.21608/ejchem.2021.90428.4315
rashad, S., abdel-tawab, F., fahmy, E., Attallah, A., Ahmed, E., haggran, A. Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast. EKB Journal Management System, 2021; 64(12): 7649-7667. doi: 10.21608/ejchem.2021.90428.4315

Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast

Egyptian Journal of Chemistry
Article 80, Volume 64, Issue 12, December 2021, Page 7649-7667  XML PDF (1.03 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.90428.4315
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Authors
shimaa elsayed rashad email orcid 1; fatthy mohamed abdel-tawab2; eman mahmoud fahmy3; Ashraf Gamil Attallah4; Ekram Sallah Eldin Ahmed5; abdelhamed abdelalem haggran6
1Genetic Engineering and Biotechnology, National Research Centre, Giza, egypt
2Department of Genetics, Faculty of Agriculture, Ain Shams University, Kaliobeya, Egypt.
3Department of Genetics, Faculty of Agriculture, Ain Shams University, Kaliobeya, Egypt
4Microbial Genetics Department, National Research Centre, Dokki, Giza, Egypt
5Cell Biology Research Dept., Genetic Engineering and Biotechnology Division, National Research Centre , Dokki , Giza
6Department of Microbial Genetic, Genetic Engineering and Biotechnology Division, National Research Center, Giza, Egypt.
Abstract
Food additives of various types are frequently used in the food sector. Their use is justified by the need to preserve, color, or sweeten a variety of foods. Despite the fact that some additives have been demonstrated to be cytotoxic, they are nevertheless utilized in practice. The effects of monosodium glutamate (MSG), sodium benzoate (SB), and saffron on several yeast haploid knockout strains were studied in this study (YKO). We used the Comet test method to find the optimum amounts at which this set of dietary additives could cause DNA damage. Three regularly used dietary additives were found to efficiently damage DNA. We also evaluated the sensitivity of higher eukaryotic cells to the genotoxic effects of these chemicals with yeast. The comet assay exhibited a better sensitivity of yeast cells, which was undeniably confirmed. The genotypes of haploid (knockout) yeast strains were chosen based on the (Clustal Omega Multiple Sequence Alignment EMBL-EBI) alignment of human and yeast gene sequence homology.
Keywords
YKO, MSG; saffron, SB, Comet assay, genotoxic
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