ANTIMUTAGENIC ACTIVITY OF BEE POLLEN AND PROPOLIS WATER EXTRACTS AGAINST CISPLATIN-INDUCED CHROMOSOMAL ABNORMALITIES IN BONE MARROW CELLS OF MICE | ||||
| Fayoum Journal of Agricultural Research and Development | ||||
| Article 21, Volume 23, Issue 1, January 2009, Page 1-12 PDF (417.94 K) | ||||
| Document Type: Research articles. | ||||
| DOI: 10.21608/fjard.2009.197032 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Amany A. Tohamy1; Ehab M. Abdella2; Rasha R. Ahmad2 | ||||
| 1Faculty of Science, Zoology Department, Helwan University, Egypt. | ||||
| 2Faculty of Science, Zoology Department, Beni-Suef University, Egypt. | ||||
| Abstract | ||||
| The protective activity of Bee-collected pollen (BPE) and watersoluble derivative of propolis (WSDPE) aqueous extracts was studied on cisplatin (CDDP) induced genotoxicity in male albino mice (Mus mascullus). The treatment of mice with Bee-collected pollen and propolis extracts at doses 140 and 8.4 mg/kg body weight/day, respectively for 14 days synergistically with the intraperitoneal administration of cisplatin at dose of 2.8 mg/kg b.wt exhibited significant chemoprotective activity. Genotoxicity and cytotoxicity were evaluated by the bone marrow chromosomal aberration assay and mitotic index, respectively. The animals of positive control group < br />(CDDP alone) showed a significant increase in genotoxicity. WSDPE and BPE, alone did not significantly induce chromosomal aberrations confirming their non-mutagenic effects. While, the animals in groups five and six (G5 and G6), that were injected i.p. with CDDP alone for one week and then for the next 14 days these animals were given WSDPE and BPE through oral intubation in synergistic with i.p. injection of CDDP, exhibited a significant decrease in cytogenetic damages induced by CDDP in bone marrow cells. The anticytotoxicity effects of WSDPE and BPE were also evident, as observed by significant increase in mitotic index, when compared to positive control group (G2). Thus, results of the present investigation revealed that WSDP and BPE have chemoprotective potentials against CDDP induced genotoxicity in bone marrow cells of male albino mice. Also, the present investigation indicated that the chemoprotective frequency of BPE was much greater than WSDPE. | ||||
| Keywords | ||||
| Antimutagen; Albino mice; Bee pollen; Propolis | ||||
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