The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 15, Volume 30, Issue 4, October 2021, Pages 121-127 PDF (519.21 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2021.197470 | ||
| Authors | ||
| Nahla Yassin sahloul* 1; Nehad Kamel2; Samira Shoeib2; Noha M. Gohar2 | ||
| 1Medical Microbiology and Immunology, Cairo University | ||
| 2Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt | ||
| Abstract | ||
| Background: Carbapenem-resistant Pseudomonas infections become a serious problem with limited options of treatment. Many studies support the role of vitamin D in competing different infections by many mechanisms through enhancing host immune defense. Objective: to assess the antimicrobial effect of vitamin D on carbapenem-resistant Pseudomonas. Methodology: Fifty carbapenem-resistant Pseudomonas isolates were studied. All isolates were tested for susceptibility to imipenem and meropenem using disk diffusion method. Modified carbapenem inactivation methods were done for carbapenemase and metallo-beta-lactamase detection. Isolates were tested for MIC of meropenem and vitamin D by broth-microdilution and their combination by checkerboard method. Results: Regarding the combination between meropenem and vitamin D; 10 isolates showed decrease in their MIC of meropenem. One isolate showed additive result, 5 isolates showed decrease more than 2 folds, 4 isolates showed 2 folds decrease. Conclusion: Vitamin D acts as an adjuvant to meropenem rather than having a direct antimicrobial effect on carbapenem-resistant Pseudomonas species. | ||
| Keywords | ||
| Pseudomonas; carbapenem resistance; meropenem; vitamin D; checkerboard | ||
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