Cytotoxicity and antimicrobial activities of some soft corals inhabiting the red sea, Egypt.
Alian, A., Abdel Shakor, A., Geba, K., Osman, A. (2021). Cytotoxicity and antimicrobial activities of some soft corals inhabiting the red sea, Egypt.. EKB Journal Management System, 2021(12), 1-7. doi: 10.21608/absb.2021.87211.1128
Abdallah Alian; Abo bakr Abdel Shakor; Khaled M. Geba; Alaa G. Osman. "Cytotoxicity and antimicrobial activities of some soft corals inhabiting the red sea, Egypt.". EKB Journal Management System, 2021, 12, 2021, 1-7. doi: 10.21608/absb.2021.87211.1128
Alian, A., Abdel Shakor, A., Geba, K., Osman, A. (2021). 'Cytotoxicity and antimicrobial activities of some soft corals inhabiting the red sea, Egypt.', EKB Journal Management System, 2021(12), pp. 1-7. doi: 10.21608/absb.2021.87211.1128
Alian, A., Abdel Shakor, A., Geba, K., Osman, A. Cytotoxicity and antimicrobial activities of some soft corals inhabiting the red sea, Egypt.. EKB Journal Management System, 2021; 2021(12): 1-7. doi: 10.21608/absb.2021.87211.1128

Cytotoxicity and antimicrobial activities of some soft corals inhabiting the red sea, Egypt.

Al-Azhar Bulletin of Science
Article 1, Volume 2021, Issue 12, December 2021, Page 1-7  XML PDF (545.57 K)
Document Type: Original Article
DOI: 10.21608/absb.2021.87211.1128
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Authors
Abdallah Alian email 1; Abo bakr Abdel Shakor2; Khaled M. Geba3; Alaa G. Osman1
1Department of Zoology, Faculty of Science, Al-Azhar University, Assiut-Branch, Assiut, Egypt.
2Laboratory of Molecular Cell Biology, Départment of Zoology, Faculty of Science, Assiut University, Assiut, Egypt.
3Molecular Biology and Biotechnology Lab, Department of Zoology, Faculty of Science, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
Abstract
 
 
Cancer and infectious diseases are notoriously known as deleterious health threats for the world, especially in the developing countries. The aim of the present study was to evaluate the cytotoxicity and antimicrobial activities of the methanolic extracts of  the soft corals Nephthea elatensis, Heteroxenia fuscescens, Ellisella juncea, Dendronephthya mollis, and Sinularia hirta, that are native to the Red Sea in Egypt. The cytotoxicity assay was carried out by the enzymatic reduction of 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay against lung adenocarcinoma cell line (A549). Moreover, the antimicrobial activity was carried out against 11 human pathogenic bacterial and fungal strains using well-cut diffusion technique, while the Minimum Inhibitory Concentrations (MICs) were determined by microdilution method. Nephthea elatensis showed potent cytotoxicity [half maximal inhibitory concentration (IC50) 11.9 ± 1.2 µg/mL]. Also, it exhibited a potent antibacterial activity against Staphylococcus aureus(MIC 1.0 µg/mL). Sinularia hirta exhibited significant antimicrobial activities against Salmonella typhimurium and S. aureus (MIC 5.0 and 10.0 µg/mL, respectively). The results recommended N. elatensis and S. hirta as promising sources for new anticancer and antibiotic natural candidates.
Keywords
Antimicrobial; Cytotoxicity; MTT, Nephthea elatensis; Sinularia hirta; Soft corals
References
[1] Houghton P, Fang R, Techatanawat I, Steventon G, Hylands PJ, Lee CC. The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity. Methods. 2007 Aug 1;42(4):377-87.

[2] Nagamalla L, Kumar JS, Sanjay C, Alsamhan AM, Shaik MR. In-silico study of seaweed secondary metabolites as AXL kinase inhibitors. Saudi Journal of Biological Sciences. 2022 Feb 1;29(2):689-701.

[3] Fares MY, Salhab HA, Khachfe HH, Khachfe HM. Breast cancer epidemiology among Lebanese women: an 11-year analysis. Medicina. 2019 Aug;55(8):463.

[4] Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathogens and global health. 2015 Oct 3;109(7):309-18.

[5] Herraiz-Carboné M, Cotillas S, Lacasa E, de Baranda CS, Riquelme E, Cañizares P, Rodrigo MA, Sáez C. A review on disinfection technologies for controlling the antibiotic resistance spread. Science of the Total Environment. 2021 Nov 25;797:149150.

[6] Adrizain R, Suryaningrat F, Alam A, Setiabudi D. Incidence of multidrug-resistant, extensively drug-resistant and pan-drug-resistant bacteria in children hospitalized at Dr. Hasan Sadikin general hospital Bandung Indonesia. InIOP Conference Series: Earth and Environmental Science 2018 Mar 1 (Vol. 125, No. 1, p. 012077). IOP Publishing.

[7] Blunt JW, Copp BR, Keyzers RA, Munroa MH, Prinsepd MR. Natural product reports. Nat Prod Rep. 2016;33:382-431.

[8] Cziesielski MJ, Duarte CM, Aalismail N, Al-Hafedh Y, Anton A, Baalkhuyur F, Baker AC, Balke T, Baums IB, Berumen M, Chalastani VI. Investing in blue natural capital to secure a future for the Red Sea ecosystems. Frontiers in Marine Science. 2021:1183...

[9] Fouad MA, Orabi MA, Abdelhamid RA, Allian A. Cytotoxicity and anti-leishmanial activity of the Red Sea soft coral Sarcophyton spongiosum. Journal of advanced Biomedical and Pharmaceutical Sciences. 2021 Apr 1;4(2):107-10.

[10] Cao F, Zhou J, Xu KX, Zhang MQ, Wang CY. New cembranoid diterpene from the South China Sea soft coral Sarcophyton sp. Natural Product Communications. 2013 Dec;8(12):1934578X1300801204..

[11] Al-Lihaibi SS, Alarif WM, Abdel-Lateff A, Ayyad SE, Abdel-Naim AB, El-Senduny FF, Badria FA. Three new cembranoid-type diterpenes from Red Sea soft coral Sarcophyton glaucum: Isolation and antiproliferative activity against HepG2 cells. European journal of medicinal chemistry. 2014 Jun 23;81:314-22.

[12] Zidan SA, Abdelhamid RA, Al-Hammady M, Fouad MA, Matsunami K, Orabi MA. Cytotoxic polyhydroxy sterols from the Egyptian Red Sea soft coral Sarcophyton acutum. Fitoterapia. 2020 Nov 1;147:104765.

[13] Zidan SA, Orabi MA, Mustafa MA, AAl-Hammady M, Kamel MS. Anti-HSV-1 and hepatoprotective activities of the Soft coral Sarcophyton acutum from the red sea. Journal of Pharmacognosy and Phytochemistry. 2016 Sep 1;5(5):247.

[14] Cao F, Zhou J, Xu KX, Zhang MQ, Wang CY. New cembranoid diterpene from the South China Sea soft coral Sarcophyton sp. Natural Product Communications. 2013 Dec;8(12):1934578X1300801204..

[15] Atlas RM. Handbook of media for environmental microbiology (p. 265, 412).

[16] Guinea J, Peláez T, Alcalá L, Bouza E. Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia. Diagnostic microbiology and infectious disease. 2005 Dec 1;53(4):333-4.

[17] Bryce M, Poliseno A, Alderslade P, Vargas S. Digitate and capitate soft corals (Cnidaria: Octocorallia: Alcyoniidae) from Western Australia with reports on new species and new Australian geographical records. Zootaxa. 2015;3963(2):160-200.

[18] K Fabricius, Alderslade P. Soft corals and sea fans: a comprehensive guide to the tropical shallow water genera of the central-west Pacific, the Indian Ocean and the Red Sea. Australian Institute of Marine Science; 2001.

[19] Fouad MA, Orabi MA, Abdelhamid RA, Allian A. Cytotoxicity and anti-leishmanial activity of the Red Sea soft coral Sarcophyton spongiosum. Journal of advanced Biomedical and Pharmaceutical Sciences. 2021 Apr 1;4(2):107-10.

[20] Khan ZA, Siddiqui MF, Park S. Current and emerging methods of antibiotic susceptibility testing. Diagnostics. 2019 Jun;9(2):49.

[21] AH Ibrahim H, S Amer M, O Ahmed H, A Hassan N. Antimicrobial activity of the sea hare (Aplysia fasciata) collected from the Egyptian Mediterranean Sea, Alexandria. Egyptian Journal of Aquatic Biology and Fisheries. 2020 Jul 1;24(4):233-48.

[22] Amer MS, Ibrahim HA. Chitosan from marine-derived Penicillum spinulosum MH2 cell wall with special emphasis on its antimicrobial and antifouling properties. The Egyptian Journal of Aquatic Research. 2019 Dec 1;45(4):359-65.

[23] MM El-Sayed W, M Elshaer M, AH Ibrahim H, EA El-Metwaly M. Antimicrobial agents from sea urchin (Diadema setosum) collected from the Red Sea, Egypt. Egyptian Journal of Aquatic Biology and Fisheries. 2020 Jul 1;24(5):33-51.

[24] Andrews JM. Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy. 2001 Jul 1;48(suppl_1):5-16.

[25] Abdelhafez OH, Fahim JR, Desoukey SY, Kamel MS, Abdelmohsen UR. Recent updates on corals from Nephtheidae. Chemistry & Biodiversity. 2019 Jun;16(6):e1800692.

[26] Abou El-Kassem LT, Hawas UW, El-Desouky SK, Al-Farawati R. Sesquiterpenes from the Saudi Red Sea: Litophyton arboreum with their cytotoxic and antimicrobial activities. Zeitschrift für Naturforschung C. 2018 Jan 1;73(1-2):9-14.

[27] Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathogens and global health. 2015 Oct 3;109(7):309-18.

[28] Ghandourah MA, Alarif WM, Abdel-Lateff A, Al-Lihaibi SS, Ayyad SE, Basaif SA, Badria FA. Two new terpenoidal derivatives: A himachalene-type sesquiterpene and 13, 14-secosteroid from the soft coral Litophyton arboreum. Medicinal Chemistry Research. 2015 Dec;24(12):4070-7.

[29] Abdel-Lateff A, Alarif WM, Al-Lihaibi SS, Abdel-Naim AB. Antiproliferative effects of selected marine organisms collected from Red Sea. Pakistan Journal of Pharmaceutical Sciences. 2017 Mar 1;30(2):381-6.

[30] Jurkevitch E, editor. Predatory prokaryotes: biology, ecology and evolution. Springer Science & Business Media; 2006 Dec 8.

[31] Amade P, Charroin C, Baby C, Vacelet J. Antimicrobial activities of marine sponges from the Mediterranean Sea. Marine Biology. 1987 Mar;94(2):271-5.

[32] Donia M, Hamann MT. Marine natural products and their potential applications as anti-infective agents. The Lancet infectious diseases. 2003 Jun 1;3(6):338-48.

[33]   Khalesi, M. K., Beeftink, R. H., & Wijffels, R. H. (2008). The soft coral Sinularia flexibilis: potential for drug development. Advances in coral husbandry in public aquariums, 2, 47e60.

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