PRODUCTION OF SOME BIODEGRADABLE POLYMERS BY SOME BACTERIAL ISOLATES
El-Sawah, M., Kassem, M., Selim, A., Ashour, E., El-Kadi, S. (2008). PRODUCTION OF SOME BIODEGRADABLE POLYMERS BY SOME BACTERIAL ISOLATES. EKB Journal Management System, 33(8), 6053-6064. doi: 10.21608/jacb.2008.200706
M.M.A. El-Sawah; M.M. Kassem; A.E.I. Selim; Eman H. Ashour; Sh.M. El-Kadi. "PRODUCTION OF SOME BIODEGRADABLE POLYMERS BY SOME BACTERIAL ISOLATES". EKB Journal Management System, 33, 8, 2008, 6053-6064. doi: 10.21608/jacb.2008.200706
El-Sawah, M., Kassem, M., Selim, A., Ashour, E., El-Kadi, S. (2008). 'PRODUCTION OF SOME BIODEGRADABLE POLYMERS BY SOME BACTERIAL ISOLATES', EKB Journal Management System, 33(8), pp. 6053-6064. doi: 10.21608/jacb.2008.200706
El-Sawah, M., Kassem, M., Selim, A., Ashour, E., El-Kadi, S. PRODUCTION OF SOME BIODEGRADABLE POLYMERS BY SOME BACTERIAL ISOLATES. EKB Journal Management System, 2008; 33(8): 6053-6064. doi: 10.21608/jacb.2008.200706

PRODUCTION OF SOME BIODEGRADABLE POLYMERS BY SOME BACTERIAL ISOLATES

Journal of Agricultural Chemistry and Biotechnology
Article 1, Volume 33, Issue 8, August 2008, Page 6053-6064  XML PDF (701.39 K)
Document Type: Original Article
DOI: 10.21608/jacb.2008.200706
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Authors
M.M.A. El-Sawah1; M.M. Kassem1; A.E.I. Selim1; Eman H. Ashour1; Sh.M. El-Kadi2
1Microbiology Dept., Fac. of Agric. Mansoura Univ., Mansoura , Egypt.
2Microbiology Dept., Damietta Fac. of Agric., Mansoura Univ., Egypt.
Abstract
Several bacterial isolates were isolated from Dakahlia and Damitta Governorates. The isolates were purified, identified and tested for PHB production. The tested isolates were 17 isolates of Azotobacter chroococcum, 8 isolates of Azospirillum lipoferum, 22 isolates of Alcaligenes eutrophus, 3 isolates of A. latus, 3isolates of Bacillus subtilis, 2isolates of B. cereus, 5 isolates of B. megaterium, 2 isolates of B. coagulans, 3 isolates of B. polymexa, 4 isolates of B. thuringiensis, 2 isolates of Pseudomonas fluorescens, 2 isolates of P. putida, 2 isolates of P. alcaligenes, 2 isolates of P. aeruginosa, 4 isolates of Rhizobium leguminosarim, 2 isolates of R. meliloti, one isolate of R. japonicum and 12 isolates of Streptomyces albus.Also, the occurrence of alginate in different isolates of Azotobacter and Pseudomonas were investigated. All isolated strains proved to be PHB producers. The amount of PHB produced by Bacillus isolates varied from 0.02 to 0.16 g/l, while by Azospirillum isolates the PHB amounts ranged from 0.12 to 0.58 g/l. Using Rhizobium isolates, the highest amount of PHB (0.36g/l) was produced by R. leguminasarim No 3 , while the lowest amount (0.06 g/l) was obtained by R. leguminosarim No 2. Usig two media, Alcaligenus eutrophus No. 20, seemed to be the most active PHB producer among all Alcaligenes isolates (0.52 g/l). By Pseudomonas isolates, Pseudomonas fluorescens No. 2 produced 0.30g/L of PHB while P. aeruginosa No. 2 produced 0.10 g/L. Generally the isolate of Azotobacter chroococcum No. 16 showed the highest values. The cell dry weight, PHB concentration and the yield of PHB were 2.28 g/l, 0.78 g/l, 34.21%, respectively. And the highest amount of alginate concentration (g/l) was 0.44 by Azotobacter chroococcum No. 14.
Keywords
Poly-β-hydroxybutyrate; alginate and bioplastic
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